Evaluation by Q-RTPCR of the efficacy of ribavirin complexed with beta-cyclodextrin against measles virus in a mouse encephalitis model
Evaluation by Q-RTPCR of the efficacy of ribavirin complexed with beta-cyclodextrin against measles virus in a mouse encephalitis model
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DOI：
10.1016/j.patbio.2006.07.008
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发表时间：
2006-12-01
影响因子：
--
通讯作者：
Venard, V.
中科院分区：
文献类型：
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作者：
Jeulin, H.;Grancher, N.;Venard, V.
以环糊精为载体，研究利巴韦林对麻疹脑炎感染的抗病毒活性。环寡糖的使用可以促进许多药物的活性，利巴韦林与β -环糊精结合的益处已经在体外得到证实。颅内接种啮齿动物适应的神经毒性麻疹病毒CAM/RB株可诱导CBA/ca小鼠脑炎。利巴韦林/ β -环糊精摩尔比为1:1的复合物抗病毒活性已在上述脑炎模型中进行了体内评估。CBA/ca小鼠；每日腹腔注射利巴韦林（40mg /kg）加或不加环糊精。实时逆转录-聚合酶链反应法测定小鼠脑内病毒载量。与蒸馏水和利巴韦林治疗相比，通过腹腔注射利巴韦林/ β -环糊精复合物（1:1）治疗小鼠，大脑中的病毒载量分别减少1.1和0.7 log(10) Eq拷贝× mL（-1）。与此同时，游离利巴韦林注射与蒸馏水处理相比，差异可以忽略不计。(c) 2006 Elsevier Masson SAS。版权所有。
The objective of this work was to study the antiviral activity of ribavirin on measles encephalitis infection when using cyclodextrins as carriers. The use of cyclic oligosaccharides can promote the activity of many drugs and the benefit of the association of ribavirin with beta-cyclodextrin has already been demonstrated in vitro. Intracranial inoculation of the rodent adapted neurovirulent CAM/RB strain of measles virus induces encephalitis in CBA/ca mice. The antiviral activity of the complex ribavirin/beta-cyclodextrinat molar ratio 1:1 has been evaluated in vivo in the above encephalitis model. CBA/ca mice were; treated with daily intraperitoneal injection of ribavirin (40 mg/kg) with or without cyclodextrin. The viral load in the brain of mice was quantified by real-time Reverse transcription-Polymerase chain reaction. Treatment of mice by the complex ribavirin/beta-cyclodextrin (1:1) by intraperitoneal route decreases the viral load in the brain of 1.1 and 0.7 log(10) Eq copies x mL(-1) compared to distillated water and ribavirin treatment, respectively. At the same time, free ribavirin injection shows a negligible difference compared to treatment by distillated water. (c) 2006 Elsevier Masson SAS. All rights reserved.