SYP-3 restricts synaptonemal complex assembly to bridge paired. chromosome axes during meiosis in Caenorhabditis elegans
SYP-3 restricts synaptonemal complex assembly to bridge paired. chromosome axes during meiosis in Caenorhabditis elegans
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DOI：
10.1534/genetics.107.072413
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发表时间：
2007-08-01
期刊：
影响因子：
3.3
通讯作者：
Colaiacovo, Monica P.
中科院分区：
文献类型：
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作者：
Smolikov, Sarit;Eizinger, Andreas;Colaiacovo, Monica P.
联会复合体(SC)的形成必须被调节为只发生在对齐的同源染色体对之间，最终确保减数分裂中适当的染色体分离。在这里，我们识别了SYP-3，这是一种螺旋卷曲蛋白，它是组装SC中心区所必需的，并限制其加载仅在适当的环境中发生，形成连接秀丽线虫减数分裂染色体对轴的结构。我们发现，中心区蛋白质的不适当加载干扰了同源基因配对，可能是通过在早期前期触发染色体配置的过早变化，从而终止对同源基因的搜索。结果，SYP-3突变体缺乏交叉，并表现出更多的染色体错误分离。综上所述，我们的研究导致我们提出SYP-3调节染色体上的突触，有助于早期前期的减数分裂进程。
Synaptonemal complex (SC) formation must be regulated to occur only between aligned pairs of homologous chromosomes, ultimately ensuring proper chromosome segregation in meiosis. Here we identify SYP-3, a coiled-coil protein that is required for assembly of the central region of the SC and for restricting its loading to occur only in an appropriate context, forming structures that bridge the axes of paired meiotic chromosomes in Caenorhabditis elegans. We find that inappropriate loading of central region proteins interferes with homolog pairing, likely by triggering a premature change in chromosome configuration during early prophase that terminates the search for homologs. As a result, syp-3 mutants lack chiasmata and exhibit increased chromosome missegregation. Altogether, our studies lead us to propose that SYP-3 regulates synapsis along chromosomes, contributing to meiotic progression in early prophase.