Human endogenous retrovirus W env increases nitric oxide production and enhances the migration ability of microglia by regulating the expression of inducible nitric oxide synthase
Human endogenous retrovirus W env increases nitric oxide production and enhances the migration ability of microglia by regulating the expression of inducible nitric oxide synthase
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人内源性逆转录病毒W env通过调节诱导型一氧化氮合酶的表达增加一氧化氮生成并增强小胶质细胞的迁移能力
DOI：
10.1007/s12250-017-3997-4
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发表时间：
2017-06-01
影响因子：
5.5
通讯作者：
Zhu, Fan
中科院分区：
文献类型：
--
作者：
Xiao, Ran;Li, Shan;Zhu, Fan
人内源性逆转录病毒W env（HERV-W env）在精神分裂症和多发性硬化症（MS）等多种神经心理疾病中起重要作用。这些疾病伴随着中枢神经系统（CNS）的免疫反应。小胶质细胞是脑炎症中重要的免疫细胞，可产生一氧化氮（NO）。NO不仅在神经细胞的功能中发挥作用，而且参与多种神经心理疾病的发病机制。在这项研究中，我们报道了CHME-5小胶质细胞转染HERV-W env后NO的产生增加。此外，HERV-W env还能增加人诱导型一氧化氮合酶（hiNOS）的表达和功能，并增强hiNOS的启动子活性。小胶质细胞迁移也增强。这些数据表明HERV-W env可能通过调节诱导型NOS的表达而促进神经心理疾病中NO的产生和小胶质细胞的迁移能力。这项研究的结果可能会导致识别新的目标，用于治疗神经心理疾病，包括神经炎性疾病，中风和神经退行性疾病。
Human endogenous retrovirus W env (HERV-W env) plays a critical role in many neuropsychological diseases such as schizophrenia and multiple sclerosis (MS). These diseases are accompanied by immunological reactions in the central nervous system (CNS). Microglia are important immunocytes in brain inflammation that can produce a gasotransmitter–nitric oxide (NO). NO not only plays a role in the function of neuronal cells but also participates in the pathogenesis of various neuropsychological diseases. In this study, we reported increased NO production in CHME-5 microglia cells after they were transfected with HERV-W env. Moreover, HERV-W env increased the expression and function of human inducible nitric oxide synthase (hiNOS) and enhanced the promoter activity of hiNOS. Microglial migration was also enhanced. These data revealed that HERV-W env might contribute to increase NO production and microglial migration ability in neuropsychological disorders by regulating the expression of inducible NOS. Results from this study might lead to the identification of novel targets for the treatment of neuropsychological diseases, including neuroinflammatory diseases, stroke, and neurodegenerative diseases.