Localization of calcitonin gene-related peptide in cardiomyocytes: comparison of neonatal and dedifferentiating cells to adult myocytes
Localization of calcitonin gene-related peptide in cardiomyocytes: comparison of neonatal and dedifferentiating cells to adult myocytes
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DOI：
10.1016/j.peptides.2004.09.007
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发表时间：
2005-02-01
期刊：
影响因子：
3
通讯作者：
Schiess, MC
中科院分区：
文献类型：
--
作者：
Bick, RJ;Poindexter, BJ;Schiess, MC
本研究的目的是定位新生儿降钙素基因相关肽结合的位点。新分离和去分化的成人心肌细胞，以帮助阐明这种神经肽的作用机制。先前的研究表明，使用降钙素基因相关肽治疗会导致钙瞬态的剧烈变化，因此我们对荧光标记的图像进行了多通道采集，以揭示降钙素基因相关蛋白和l型钙通道的定位。降钙素基因相关蛋白在棒状成人心肌细胞中稀疏且随机分布，在细胞条纹区大量存在；在去分化的成人肌细胞中，黏附蛋白占主导地位，在核周浓度很大，在新生细胞中，一些黏附蛋白扩散到细胞质中。随后的建模表明，降钙素基因相关肽和l型钙通道蛋白在三种心肌细胞类型中都密切相关。这表明，虽然肽对不同心肌细胞的细胞内钙水平有显著而不同的影响，但由于细胞内钙浓度的改变，其作用可能通过不同的机制作用于不同的通道或泵蛋白。(C) 2004爱思唯尔公司版权所有。
The purpose of this study was to localize sites of calcitonin gene-related peptide binding in neonatal. freshly isolated and dedifferentiated adult cardiac myocytes in order to help Lis elucidate the mechanisms of action of this neuropeptides. Previous work has shown that treatment with calcitonin gene-related peptide results in dramatic changes in calcium transients, so we carried out multi-channel acquisitions of fluorescently labeled images to reveal where calcitonin gene-related protein and the L-type calcium channel were localized. Calcitonin gene-related protein was sparse and randomly distributed in rod-like adult cardiomyocytes, found in abundance in areas of the cell where striations; were apparent and not where adhesion proteins predominated in dedifferentiating adult myocytes, and in a large perinuclear concentration, with some spreading into the cytoplasm in neonatal cells. Subsequent modeling demonstrated that calcitonin gene-related peptide and the L-type calcium channel protein were closely associated in each of the three myocyte types. suggesting that while the peptide has dramatic and different effects on intracellular calcium levels of the various cardiomyocytes, the action is probably via diverse mechanisms as a result of effects on different channels or pump proteins due to alterations in intracellular calcium Concentrations. (C) 2004 Elsevier Inc. All rights reserved.