Particle-cell dynamics in human blood flow: Implications for vascular-targeted drug delivery
Particle-cell dynamics in human blood flow: Implications for vascular-targeted drug delivery
复制标题
DOI：
10.1016/j.jbiomech.2012.08.035
复制
发表时间：
2012-11-15
影响因子：
2.4
通讯作者：
Eniola-Adefeso, Omolola
中科院分区：
文献类型：
--
作者：
Charoenphol, Phapanin;Onyskiw, Peter J.;Eniola-Adefeso, Omolola
血管靶向治疗的结果通常取决于血管靶向载体在靶向部位定位和粘附到内皮壁的效率。本研究调查了白细胞、血小板和红细胞在各种生理血流条件下对血管靶向聚合物纳米球和微球的边缘化的影响。我们报告，红细胞促进或阻碍颗粒粘附到内皮细胞壁在平行板流动室取决于血流模式，红细胞压积，和颗粒大小。白细胞通过（1）竞争可用的结合空间和（2）物理去除先前结合的球体，防止微球（而不是纳米球）粘附在层流和脉动流中。在再循环血流中，白细胞对颗粒粘附的负面影响是最小的大微球在扰动流区超出流动再附着。发现静息血小板对颗粒结合没有影响，可能是由于其尺寸和与内皮壁的最小相互作用。总体而言，本工作的发现对于设计有效的血管靶向载体用于几种人类疾病的成像和药物递送应用至关重要。(C)2012爱思唯尔有限公司版权所有。
The outcome of vascular-targeted therapies is generally determined by how efficiently vascular-targeted carriers localize and adhere to the endothelial wall at the targeted site. This study investigates the impact of leukocytes, platelets and red blood cells on the margination of vascular-targeted polymeric nanospheres and microspheres under various physiological blood flow conditions. We report that red blood cells either promote or hinder particle adhesion to an endothelial wall in a parallel plate flow chamber depending on the blood flow pattern, hematocrit, and particle size. Leukocytes prevent microspheres - but not nanospheres - from adhering in laminar and pulsatile flows via (1) competition for the available binding space and (2) physical removal of previously bound spheres. In recirculating blood flow, the negative effect of leukocytes on particle adhesion is minimal for large microspheres in the disturbed flow region beyond the flow reattachment. Resting platelets were found to have no effect on particle binding likely due to their dimensions and minimal interaction with the endothelial wall. Overall, the findings of the present work would be critical for designing effective vascular-targeted carriers for imaging and drug delivery applications in several human diseases. (C) 2012 Elsevier Ltd. All rights reserved.