A non-enzymatic nanomagnetic electro-immunosensor for determination of Aflatoxin B1 as a model antigen
A non-enzymatic nanomagnetic electro-immunosensor for determination of Aflatoxin B1 as a model antigen
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DOI：
10.1016/j.snb.2012.11.067
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发表时间：
2013-02-01
影响因子：
8.4
通讯作者：
Davarani, Saied Saeed Hosseiny
中科院分区：
文献类型：
--
作者：
Masoomi, Leila;Sadeghi, Omid;Davarani, Saied Saeed Hosseiny
采用改性玻璃碳电极：GCE/壳聚糖/金纳米颗粒(AuNP)/抗黄曲霉毒素B-1 （AFB(1)）和3-（（2-巯基乙基）甲基）苯-1,2-二醇功能化的Fe3O4磁芯，并用AFB(1)标记，研制了一种非酶三明治型电化学免疫传感器。利用该免疫传感器检测黄曲霉毒素B-1作为模型抗原。电流来源于邻苯二酚和邻醌在准可逆的两个电子-两个质子反应中的转化，被认为是响应。电化学研究表明，该免疫电极可用于检测范围为0.6 ~ 110 ng mL（-1）的AFB(1)，检测限为0.2 ng mL（-1）。这种免疫传感器可以简单地通过使用外部磁场再生，以确保可重复的响应。(c) 2012 Elsevier B.V.版权所有
A nonenzymatic sandwich-type electrochemical immunosensor was developed by means of a modified glassy carbon electrode: GCE/chitosan/Au nanoparticle (AuNP)/anti-Aflatoxin B-1 (a AFB(1)), and a Fe3O4 magnetic core with a gold shell functionalized with 3-((2-mercaptoethylimino)methyl)benzene-1,2-diol and labeled with an AFB(1). The immunosensor was used for the detection of Aflatoxin B-1 as a model antigen. The current originated from the conversion of catechol to o-quinone and vice versa within a quasi-reversible two electrons-two protons reaction, considered as the response. The electrochemical studies demonstrated that the proposed immunoelectrode can be used to detect AFB(1) in the range of 0.6-110 ng mL(-1) with a detection limit of 0.2 ng mL(-1). This immunosensor can be simply regenerated by using an external magnetic field which ensures a reproducible response. (c) 2012 Elsevier B.V. All rights reserved.