NEURONAL ORIGIN OF BOMBESIN-LIKE IMMUNOREACTIVITY IN THE RAT GASTRO-INTESTINAL TRACT
NEURONAL ORIGIN OF BOMBESIN-LIKE IMMUNOREACTIVITY IN THE RAT GASTRO-INTESTINAL TRACT
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DOI：
10.1016/0306-4522(79)90019-8
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发表时间：
1979-01-01
期刊：
影响因子：
3.3
通讯作者：
WALSH, JH
中科院分区：
文献类型：
--
作者：
DOCKRAY, GJ;VAILLANT, C;WALSH, JH
在放射免疫分析中，大鼠胃的肌肉和粘膜层的提取物中含有与两栖动物[Bombina Bombina]皮肤多肽蛙皮素发生交叉反应的物质。在肠道中，免疫反应阳性的蛙皮素仅限于肌层。鉴定了两种具有免疫反应活性的分子形式；其中一种在凝胶过滤中与十四肽蛙素的洗脱位置相似，约占肠道提取物中免疫活性的90%，而在胃中约占40%。根据它们与3种不同抗血清的免疫化学性质模式，这两个组分可与合成蛙皮素和结构相关的多肽P物质区分开来。用相同的抗血清进行免疫组织化学研究发现，大鼠胃黏膜中神经纤维分布丰富，而肠粘膜中神经纤维很少。在整个肠道肌间神经丛的神经细胞体周围发现了丰富的具有蛙皮素样免疫反应的纤维。免疫反应阳性的神经细胞体没有被鉴定，也没有令人信服的证据表明蛙皮素存在于粘膜内分泌细胞中。蛙皮素样肽可能是肠道内的神经递质，在调节胃肠动力和胃泌素释放方面发挥作用。
Extracts of muscle and mucosal layers of the rat stomach contained material cross-reacting in radioimmunoassays for the amphibian [Bombina bombina] skin peptide bombesin. In the intestine, immunoreactive bombesin was confined to the muscle layers. Two molecular forms of immunoreactive bombesin were identified; one of these components was eluted in a similar position to tetradecapeptide bombesin on gel filtration and accounted for about 90% of the immunoreactivity in intestinal extracts, compared with about 40% in the stomach. The 2 components were distinguishable from synthetic bombesin and from the structurally related peptide substance P on the basis of their pattern of immunochemical properties with 3 different antisera. Immunohistochemical studies using the same antisera revealed a rich distribution of nerve fibers in the mucosa of the rat stomach, but few fibers were seen in the intestinal mucosa. Abundant fibers with bombesin-like immunoreactivity were found surrounding nerve cell bodies in the myenteric plexus throughout the gut. Immunoreactive nerve cell bodies were not identified, neither was convincing evidence obtained to indicate the presence of bombesin in mucosal endocrine cells. Bombesin-like peptides may be neurotransmitters in the gut and could play a role in the modulation of gastrointestinal motility and in the release of gastrin.