In vivo assessment of the effect of controlled high- and low-frequency mechanical loading on peri-implant bone healing
In vivo assessment of the effect of controlled high- and low-frequency mechanical loading on peri-implant bone healing
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DOI：
10.1098/rsif.2011.0820
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发表时间：
2012-07
影响因子：
3.9
通讯作者：
Xiaolei Zhang;K. Vandamme;A. Torcasio;Toru Ogawa;G. H. van Lenthe;I. Naert;J. Duyck
中科院分区：
文献类型：
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作者：
Xiaolei Zhang;K. Vandamme;A. Torcasio;Toru Ogawa;G. H. van Lenthe;I. Naert;J. Duyck
本研究的目的是探讨控制高频（HF）和低频（LF）机械负荷对种植体周围骨愈合的影响。在69只成年Wistar大鼠的双胫骨内植入定制的钛种植体。对于每只动物，一个植入物通过胫骨轴受压加载（试验），而另一个被卸载（对照组）。试验种植体随机分为四组，采用离体校准法确定不同的加载方式。在HF （40 Hz）或LF （2 Hz）加载类别中，分别选择低（LM）和高（HM）的幅度，从而使两个频率组的应变速率幅度恒定。这导致了四种加载方式：(i) HF-LM (40 Hz-0.5 N)；（ii） HF-HM (40hz - 1 N)；（iii） LF-LM (2 Hz-10 N)；（iv） LF-HM （2hz - 20n）加载。每周加载5次，持续1或4周。在皮质和髓质水平对组织样本进行组织学和组织形态学处理（骨与种植体接触，BIC；种植体周围骨分数，BF）。数据采用方差分析和配对t检验进行统计学分析，显著性水平为0.05。在为期一周的实验中，仅由LF-HM加载模式（2 Hz-20 N）诱导植入物表面附近皮质水平的BF增加。在高负荷- lm负荷（40赫兹- 0.5 N）和低负荷- hm负荷（2赫兹- 20 N）的情况下，四周的负荷对BIC（而不是对BF）产生了显著影响：在两种负荷下，皮层水平的BIC显著增加，而髓质水平的BIC仅在高负荷- lm负荷的情况下受到积极影响。在HF和LF的机械载荷影响骨整合和种植体周围BF。与HF载荷相比，LF载荷下需要更高的载荷强度（以及伴随的组织应变升高）才能引起种植体周围的积极骨反应。需要在HF处持续一段时间的载荷才能导致整体骨整合的增强。
The aim of this study was to investigate the effect of controlled high- (HF) and low-frequency (LF) mechanical loading on peri-implant bone healing. Custom-made titanium implants were inserted in both tibiae of 69 adult Wistar rats. For every animal, one implant was loaded by compression through the axis of tibia (test), whereas the other one was unloaded (control). The test implants were randomly distributed among four groups receiving different loading regimes, which were determined by ex vivo calibration. Within the HF (40 Hz) or LF (2 Hz) loading category, the magnitudes were chosen as low- (LM) and high-magnitude (HM), respectively, leading to constant strain rate amplitudes for the two frequency groups. This resulted in the four loading regimes: (i) HF-LM (40 Hz–0.5 N); (ii) HF-HM (40 Hz–1 N); (iii) LF-LM (2 Hz–10 N); and (iv) LF-HM (2 Hz–20 N) loading. Loading was performed five times per week and lasted for one or four weeks. Tissue samples were processed for histology and histomorphometry (bone-to-implant contact, BIC; and peri-implant bone fraction, BF) at the cortical and medullar level. Data were analysed statistically with ANOVA and paired t-tests with the significance level set at 0.05. For the one-week experiments, an increased BF adjacent to the implant surface at the cortical level was exclusively induced by the LF-HM loading regime (2 Hz–20 N). Four weeks of loading resulted in a significant effect on BIC (and not on BF) in case of HF-LM loading (40 Hz–0.5 N) and LF-HM loading (2 Hz–20 N): BIC at the cortical level significantly increased under both loading regimes, whereas BIC at the medullar level was positively influenced only in case of HF-LM loading. Mechanical loading at both HF and LF affects osseointegration and peri-implant BF. Higher loading magnitudes (and accompanying elevated tissue strains) are required under LF loading to provoke a positive peri-implant bone response, compared with HF loading. A sustained period of loading at HF is needed to result in an overall enhanced osseointegration.