A novel transforming growth factor-β superfamily member expressed in gonadal somatic cells enhances primordial germ cell and spermatogonial proliferation in rainbow trout (Oncorhynchus mykiss)
A novel transforming growth factor-β superfamily member expressed in gonadal somatic cells enhances primordial germ cell and spermatogonial proliferation in rainbow trout (Oncorhynchus mykiss)
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DOI：
10.1016/j.ydbio.2006.10.001
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发表时间：
2007-01-01
影响因子：
2.7
通讯作者：
Yoshizaki, Goro
中科院分区：
文献类型：
--
作者：
Sawatari, Etsuko;Shikina, Shinya;Yoshizaki, Goro
我们对鱼类原始生殖细胞(PGC)增殖的分子机制了解很少，但被认为是由周围的体细胞控制的。我们假设与PGC增殖相关的生长因子主要在周围的生殖器脊体细胞中表达。为了分离这些生长因子，我们构建了一个互补的DNA(CDNA)消减文库，该文库以40-DPF虹鱼胚胎生殖脊的cDNA为测试子，以无生殖脊的胚胎的cDNA为驱动子。这种方法发现了一种新的细胞因子，命名为性腺体细胞衍生生长因子(GSDF)，它是转化生长因子(TGF)-β超家族的成员。在胚胎发生过程中，GSDF在生殖器脊体细胞周围的体细胞中表达，在胚胎发育后期的颗粒细胞和支持细胞中也有表达。反义寡核苷酸抑制GSDF翻译可抑制PGC增殖。此外，用重组GSDF培养的分离的睾丸细胞表现出剂量依赖性的A型精原细胞增殖，这种作用可被抗GSDF的血清完全阻断。这些结果表明，GSDF是转化生长因子-β超家族中的一个新成员，在PGC和精原细胞的增殖中起重要作用。(C)2006 Elsevier Inc.保留所有权利。
Our understanding of the molecular mechanisms of primordial germ cell (PGC) proliferation in fish is rudimentary, but it is thought to be controlled by the surrounding somatic cells. We assumed that growth factors that are specifically involved in PGC proliferation are expressed predominantly in the surrounding genital ridge somatic cells. In order to isolate these growth factors, we compiled a complementary DNA (cDNA) subtractive library using cDNA from the genital ridges of 40-dpf rainbow trout embryos as the tester and cDNA from embryos without genital ridges as the driver. This approach identified a novel cytokine, designated gonadal soma-derived growth factor (GSDF), which is a member of the transforming growth factor (TGF)-beta superfamily. GSDF was expressed in the genital ridge somatic cells surrounding the PGCs during embryogenesis, and in both the granulosa and Sertoli cells at later stages. Inhibition of GSDF translation by antisense oligonucleotides suppressed PGC proliferation. Moreover, isolated testicular cells that were cultured with recombinant GSDF demonstrated dose-dependent proliferation of type-A spermatogonia; this effect was completely blocked by antiserum against GSDF. These results denote that GSDF, a novel member of the TGF-beta superfamily, plays an important role for proliferation of PGC and spermatogonia. (c) 2006 Elsevier Inc. All rights reserved.