A micromachined device provides a new bend on fibroblast traction forces
A micromachined device provides a new bend on fibroblast traction forces
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DOI：
10.1073/pnas.94.17.9114
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发表时间：
1997-08-19
影响因子：
11.1
通讯作者：
Sheetz, MP
中科院分区：
文献类型：
--
作者：
Galbraith, CG;Sheetz, MP
我们使用一种新型的微机械装置测量了成纤维细胞产生的牵引力，该装置能够确定单个粘附接触产生的亚细胞力。迁移的成纤维细胞的前部产生间歇性向后的力，而尾部产生较大的向前的力，没有一个力是稳定的;它们都有周期性的波动，前向和后向牵引力之间的转换发生在细胞核，而不是在细胞的后部或内外质之间的边界，我们建议，耦合板延伸到波动向后牵引在前面的核区域向前移动成纤维细胞的前面，而力促进释放后粘合剂接触和前向牵引允许核后面的区域前进。
We have measured the traction forces generated by fibroblasts using a novel micromachined device that is capable of determining the subcellular forces generated by individual adhesive contacts, The front of migrating fibroblasts produced intermittent rearward forces whereas the tail produced larger forward directed forces, None of the forces were steady; they all had periodic fluctuations, The transition between forward and rearward traction forces occurred at the nucleus, not at the rear of the cell or the border between the endoplasm and the ectoplasm, We propose that the coupling of lamella extensions to fluctuating rearward tractions in front of the nuclear region move the front of a fibroblast forward, while force-facilitated release of rear adhesive contacts and anterior-directed tractions allow the region behind the nucleus to advance.