An envelope glycoprotein of the human endogenous retrovirus HERV-W is expressed in the human placenta and fuses cells expressing the type D mammalian retrovirus receptor
An envelope glycoprotein of the human endogenous retrovirus HERV-W is expressed in the human placenta and fuses cells expressing the type D mammalian retrovirus receptor
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DOI：
10.1128/jvi.74.7.3321-3329.2000
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发表时间：
2000-04-01
影响因子：
5.4
通讯作者：
Cosset, FL
中科院分区：
文献类型：
--
作者：
Blond, JL;Lavillette, D;Cosset, FL
最近描述了一种新的人内源性逆转录病毒 (HERV) 家族，称为 HERV-W (J.-L. Blond, F. Beseme, L. Duret, O. Bouton, F, Bedin, H. Perron, B. Mandrand, and F, Mallet, J, Virol. 73:1175-1185, 1999)，发现 HERV-W mRNA 在胎盘中特异性表达细胞，并回收含有完整开放阅读框的 env cDNA。在细胞-细胞融合测定中，我们在此证明 HERV-W env 基因的产物是一种高度融合的膜糖蛋白。在一组来自不同物种的细胞系中转染 HERV-W Env 表达载体，在与 D 型哺乳动物逆转录病毒受体相互作用后，导致灵长类和猪细胞中合胞体的形成。此外，在胎盘细胞中特异性检测到了HERV-W编码的包膜糖蛋白，表明它们可能在妊娠和胎盘形成过程中发挥生理作用。
A new human endogenous retrovirus (HERV) family, termed HERV-W, was recently described (J.-L. Blond, F. Beseme, L. Duret, O. Bouton, F, Bedin, H. Perron, B. Mandrand, and F, Mallet, J, Virol. 73:1175-1185, 1999), HERV-W mRNAs were found to be specifically expressed in placenta cells, and an env cDNA containing a complete open reading frame was recovered. In cell-cell fusion assays, we demonstrate here that the product of the HERV-W env gene is a highly fusogenic membrane glycoprotein, Transfection of an HERV-W Env expression vector in a panel of cell lines derived from different species resulted in formation of syncytia in primate and pig cells upon interaction with the type D mammalian retrovirus receptor. Moreover, envelope glycoproteins encoded by HERV-W were specifically detected in placenta cells, suggesting that they may play a physiological role during pregnancy and placenta formation.