Utility of a Dengue-Derived Monoclonal Antibody to Enhance Zika Infection In Vitro.
Utility of a Dengue-Derived Monoclonal Antibody to Enhance Zika Infection In Vitro.
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DOI：
10.1371/currents.outbreaks.4ab8bc87c945eb41cd8a49e127082620
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发表时间：
2016-07-05
期刊：
影响因子：
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通讯作者：
Christofferson, Rebecca C
中科院分区：
文献类型：
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作者：
Charles, Anu Susan;Christofferson, Rebecca C
引言：寨卡病毒（ZIKV）出现在登革热（DENV）流行地区，在这些地区这两种相关的黄病毒持续共同传播。登革热病毒有四种血清型，感染可能发展为重症疾病。据认为，这是由抗体依赖增强作用（ADE）介导的，即初次登革热病毒感染产生的抗体无法中和由另一种血清型引起的异源登革热病毒感染。在黄病毒组的其他成员中也已证实存在抗体依赖增强作用。
方法：我们利用为登革热病毒开发的体外抗体依赖增强作用检测方法，来确定一种常见的源自登革热病毒血清型2的单克隆抗体（4G2）是否会增强寨卡病毒。
结果：我们表明在4G2单克隆抗体存在的情况下，体外寨卡病毒感染会增强。
讨论：我们的结果表明寨卡病毒和登革热病毒之间可能存在抗体依赖增强作用，并且4G2抗体是研究寨卡病毒感染期间预先存在的抗登革热病毒抗体影响的有用工具。
INTRODUCTION: Zika virus (ZIKV) has emerged in dengue (DENV) endemic areas, where these two related flaviviruses continue to co-circulate. DENV is a complex of four serotypes and infections can progress to severe disease. It is thought that this is mediated by antibody dependent enhancement (ADE) whereby antibodies from a primary DENV infection are incapable of neutralizing heterologous DENV infections with another serotype. ADE has been demonstrated among other members of the Flavivirus group.METHODS: We utilize an in vitro ADE assay developed for DENV to determine whether ZIKV is enhanced by a commonly available DENV serotype 2-derived monoclonal antibody (4G2).RESULTS: We show that ZIKV infection in vitro is enhanced in the presence of the 4G2 mAb.DISCUSSION: Our results demonstrate that ADE between ZIKV and DENV is possible and that the 4G2 antibody is a useful tool for the effects of pre-existing anti-DENV antibodies during ZIKV infections.