CELLULAR-LOCALIZATION AND REGULATION OF GLUTAMINE-SYNTHETASE IN PRIMARY CULTURES OF BRAIN-CELLS FROM NEWBORN MICE
CELLULAR-LOCALIZATION AND REGULATION OF GLUTAMINE-SYNTHETASE IN PRIMARY CULTURES OF BRAIN-CELLS FROM NEWBORN MICE
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DOI：
10.1111/j.1471-4159.1981.tb05289.x
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发表时间：
1981-01-01
影响因子：
4.7
通讯作者：
HAMPRECHT, B
中科院分区：
文献类型：
--
作者：
HALLERMAYER, K;HARMENING, C;HAMPRECHT, B
用间接免疫荧光法测定了新生小鼠离体脑细胞中谷氨酰胺合成酶的细胞分布。大约40%的细胞中检测到该酶，其中星形细胞形态的细胞被清楚地鉴定出来。用糖皮质激素地塞米松治疗导致阳性染色细胞数量的显著增加。在0.1 μ m浓度下，地塞米松对酶的诱导作用在36 h后达到最大。在这些条件下，谷氨酰胺合成酶比活性提高了约6倍。皮质类固醇以外的类固醇激素没有效果。这些培养物中的基础活性接近新生小鼠大脑中的活性，但远低于成年小鼠大脑中的活性，这表明在培养物中这些细胞的正常发育受到了干扰。神经胶质细胞系和神经元细胞系的比较表明，谷氨酰胺合成酶存在于两种类型的细胞系中，其比活性非常低。地塞米松对该酶的诱导作用是在胶质细胞切割中发现的，而不是在神经细胞系中。
The cellular distribution of glutamine synthetase was determined by indirect immunofluorescence in cultures of dissociated brain cells from newborn mice. The enzyme could be detected in about 40% of all cells, among which cells with astrocytic morphology were clearly identified. Treatment with the glucocorticoid dexamethasone led to a strong increase in the number of positively stained cells. Enzyme induction by dexamethasone was maximal after 36 h and at a concentration of 0.1 .mu.M. Under these conditions glutamine synthetase specific activity was elevated about 6-fold. Steroid hormones other than corticosteroids had no effect. The basal activity in these cultures was near that found in brains of newborn mice, but far below the activity in adult brains, showing that in culture the normal development of these cells is disturbed. A comparison of glial and neuronal cell lines showed that glutamine synthetase is present in both types of cell lines at a very low specific activity. Inducibility of this enzyme by dexamethasone was found in glial cut not in neuronal cell lines.