Relationship between p62 and p56, two proteins of the mammalian cortical granule envelope, and hyalin, the major component of the echinoderm hyaline layer, in hamsters.
Relationship between p62 and p56, two proteins of the mammalian cortical granule envelope, and hyalin, the major component of the echinoderm hyaline layer, in hamsters.
复制标题
仓鼠中哺乳动物皮质颗粒包膜的两种蛋白质 p62 和 p56 与棘皮动物透明层的主要成分透明蛋白之间的关系。
DOI：
10.1095/biolreprod62.4.979
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发表时间：
2000
影响因子：
3.6
通讯作者：
Talbot,P
中科院分区：
文献类型：
--
作者：
Hoodbhoy,T;CarrollJr,EJ;Talbot,P
哺乳动物皮质颗粒含有两种多肽（p62和p56），它们在受精后被掺入皮质颗粒包膜中，并在受精卵和植入前卵裂球的分裂中起作用。由于棘皮动物透明层和哺乳动物皮质颗粒包膜是类似的，并且由于透明层蛋白，透明蛋白，在早期棘皮动物胚胎发生中起作用，因此进行本研究以确定p62和p56和/或哺乳动物皮质颗粒包膜的其他组分是否与透明蛋白相关。纯化的S.通过共聚焦扫描激光显微镜显示Purpuratushyalin结合仓鼠皮质颗粒和受精仓鼠卵母细胞的皮质颗粒包膜和植入前胚胎直至胚泡阶段。在免疫印迹中，IL 2仅结合到62-和56-kDa的皮质颗粒蛋白，这些蛋白在受精后被并入皮质颗粒包膜。IL 2结合抗原似乎是由植入前胚胎在2-细胞发育阶段开始重新合成的。在体内治疗2细胞期仓鼠胚胎与IL 2抑制卵裂球分裂，但桑椹胚的治疗没有抑制囊胚植入。这些结果支持了哺乳动物皮层颗粒包膜蛋白p62/p56与棘皮动物透明蛋白具有共同抗原表位的观点，并且p62/p56与透明蛋白一样，在早期胚胎发生中发挥作用。
Mammalian cortical granules contain two polypeptides (p62 and p56) that are incorporated into the cortical granule envelope after fertilization and function in cleavage of the zygote and the preimplantation blastomeres. Since the echinoderm hyaline layer and mammalian cortical granule envelope are analogous, and since the hyaline layer protein, hyalin, functions in early echinoderm embryogenesis, this study was done to determine whether p62 and p56 and/or other components of the mammalian cortical granule envelope are related to hyalin. A polyclonal antibody (IL2) against purifiedS. purpuratushyalin was shown by confocal scanning laser microscopy to bind to hamster cortical granules and to the cortical granule envelope of fertilized hamster oocytes and preimplantation embryos up to the blastocyst stage. In immunoblots, IL2 bound only to 62- and 56-kDa cortical granule proteins that were incorporated into the cortical granule envelope after fertilization. IL2 binding antigens appeared to be resynthesized by preimplantation embryos starting at the 2-cell stage of development. In vivo treatment of 2-cell-stage hamster embryos with IL2 inhibited blastomere cleavage, but treatment of morulae did not inhibit blastocyst implantation. These results support the idea that the mammalian cortical granule envelope proteins, p62/p56, share a common antigenic epitope(s) with echinoderm hyalin, and that p62/p56, like hyalin, play a role in early embryogenesis.