Immunocytochemical localization of alpha-lactalbumin in the male reproductive tract.
Immunocytochemical localization of alpha-lactalbumin in the male reproductive tract.
复制标题
男性生殖道中α-乳清蛋白的免疫细胞化学定位。
DOI：
10.1095/biolreprod30.1.171
复制
发表时间：
1984
影响因子：
3.6
通讯作者：
Dym,M
中科院分区：
文献类型：
--
作者：
Byers,SW;Qasba,PK;Paulson,HL;Dym,M
描述了乳蛋白α-乳白蛋白在男性生殖道中的免疫细胞化学定位。使用针对高度纯化的大鼠乳α-乳白蛋白产生的初级抗血清，在近端附睾头主细胞的核上高尔基体区域中一致地显示出特异性染色，但仅偶尔在来自导管其他区域的上皮细胞中显示出特异性染色。在附睾管腔中也发现了与精子相关的染色。这种管腔染色持续存在于整个远端头、体和附睾尾部。染色很少与传出管和起始段中的精子相关。在生精上皮中也检测到了α-乳清蛋白免疫反应性。染色仅限于精子细胞的高尔基体顶体区域。这些结果表明，一种或多种α-乳清蛋白样分子存在于男性生殖道中，并且其特异性地定位于来自附睾近端的主细胞和来自生精上皮的生殖细胞中。
The immunocytochemical localization of the milk protein alpha-lactalbumin in the male reproductive tract is described. Using a primary antiserum raised against highly purified rat milk alpha-lactalbumin, specific staining was consistently shown in the supranuclear Golgi region of the principal cells of the proximal caput epididymidis but only occasionally in epithelial cells from other regions of the duct. Staining was also found in the epididymal lumen and associated with spermatozoa. This luminal staining persisted throughout the distal caput, corpus and cauda epididy-midis. Staining was rarely associated with spermatozoa in the efferent ducts and initial segment. Alpha-lactalbumin immunoreactivity was also detected in the seminiferous epithelium. Staining was confined to the Golgi-acrosome region of spermatids. These results indicate that an alphalactalbumin-like molecule, or molecules, is present in the male reproductive tract and that it is localized specifically in principal cells from the proximal caput epididymidis and germ cells from the seminiferous epithelium.