Molecular characterization of clinical Saccharomyces cerevisiae isolates and their association with non-clinical strains
Molecular characterization of clinical Saccharomyces cerevisiae isolates and their association with non-clinical strains
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DOI：
10.1078/0723202041438473
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发表时间：
2004-08-01
影响因子：
3.4
通讯作者：
Fernández-Espinar, MT
中科院分区：
文献类型：
--
作者：
de Llanos, R;Querol, A;Fernández-Espinar, MT
我们评估了96株临床分离的沙门氏菌的分子特征。从西班牙的一家医疗机构分离的酿酒酵母，并将它们与从葡萄酒、啤酒和面包中分离的6个非临床菌株和1个S.从商业制剂中收集的布拉氏菌菌株。用HinfI酶切分析和PCR扩增8个序列。虽然这两种技术都适用于常规临床分析，但基于PCR的技术被证明是最具鉴别力的。这项研究，除了提供临床应用的工具，处理临床和非临床菌株之间的关系。分析的两种面包酵母与一组31个临床分离株具有共同的线粒体DNA和PCR模式。菌株的外源性进入也反映在19个临床分离株和治疗性菌株S。布拉地。面包酵母和S.布拉氏菌分别占32.3%和19.8%，它们在人体内的定植能力和引起阴道感染的能力之间似乎有联系。其余食物分离株未与临床菌株分组。
We assessed the molecular characterization of 96 clinical isolates of S. cerevisiae from a Spanish medical institution and we compared them with 6 non-clinical strains isolated from wine, beer and bread and 1 S. boulardii strain collected from a commercial preparation. The strains were subjected to HinfI mtDNA restriction analysis and PCR amplification of 8 sequences. Although both techniques are appropriate for routine clinical analysis, that based on PCR turned out to be the most discriminating. This study, apart from providing tools for clinical application, deals with the relationships between clinical and non-clinical strains. The two baker's yeasts analysed shared mtDNA and PCR patterns with a group of 31 clinical isolates. An exogenous entry of a strain was also reflected in the case of 19 clinical isolates and the therapeutic strain S. boulardii. Both baker's yeasts and S. boulardii were identified respectively among 32.3% and 19.8% of the clinical isolates and there seemed to be a connection between their ability to colonize humans and their ability to cause vaginal infection. The rest of food isolates were not grouped with clinical strains.