Total luminescence spectral characteristics of natural organic matter (NOM) size fractions as defined by ultrafiltration and high performance size exclusion chromatography (HPSEC)
Total luminescence spectral characteristics of natural organic matter (NOM) size fractions as defined by ultrafiltration and high performance size exclusion chromatography (HPSEC)
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DOI：
10.1016/s0146-6380(02)00032-3
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发表时间：
2002-01-01
影响因子：
3
通讯作者：
Egeberg, PK
中科院分区：
文献类型：
--
作者：
Alberts, JJ;Takács, M;Egeberg, PK
通过反渗透从挪威和美国乔治亚州不同气候条件和有机源材料的两种地表水中分离出天然有机物（NOM）。通过超滤和高性能尺寸排阻色谱 (HPSEC) 技术将 NOM 分离成方法上确定的尺寸级分，并使用紫外-可见光和总发光光谱技术对这些级分进行分析。虽然观察到一些系统差异，但两个系统的 NOM 具有相似的 UV 比吸光度值，并且在较大分子尺寸部分中均具有较高的 254 nm 比吸光度。同样，两个系统的 NOM 在大约 lambda(ex)/lambda(em) = 225/425 nm 和 lambda(ex)/lambda(em) 335/445 nm 区域均具有荧光光谱峰，与淡水环境中腐殖质的光谱一致。这些在两个显着不同的环境中进行的测量以及与之前发表的文献的一致性表明，尽管材料的来源和植物材料可能接触的早期成岩过程存在差异，但淡水 NOM 相对于其吸光度和荧光发色团非常一致。 (C) 2002 Elsevier Science Ltd. 保留所有权利。
Natural organic matter (NOM) was isolated by reverse osmosis from two surface waters of differing climatic regimes and organic source material, in Norway and Georgia, USA. The NOM was separated into methodologically defined size fractions by ultrafiltration and high performance size exclusion chromatographic (HPSEC) techniques and the fractions were analyzed with UV-visible and total luminescence spectral techniques. While some systematic variation was observed, NOM from both systems had similar UV specific absorbance values and both had higher 254 nm specific absorbances in the larger molecular size fractions. Similarly, NOM from both systems had fluorescence spectral peaks in the regions of approximately lambda(ex)/lambda(em) = 225/425 nm and lambda(ex)/lambda(em) 335/445 nm, consistent with spectra of humic substances in freshwater environments. Agreement of these measurements in two significantly different environments and with previously published literature indicates that freshwater NOM is very consistent relative to its absorbance and fluorescence chromophores, despite differences in the origin of the material and the early diagenetic processes to which plant material may be exposed. (C) 2002 Elsevier Science Ltd. All rights reserved.