Chemopreventive activity of Tualang honey against oral squamous cell carcinoma-in vivo
Chemopreventive activity of Tualang honey against oral squamous cell carcinoma-in vivo
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DOI：
10.1016/j.oooo.2020.01.009
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发表时间：
2020-05-01
影响因子：
2.9
通讯作者：
Seyedan, Atefehalsadat
中科院分区：
文献类型：
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作者：
Al-koshab, May;Alabsi, Aied M.;Seyedan, Atefehalsadat
Objective.本研究的目的是评估化学预防活性的马来西亚丛林土阿朗蜂蜜（TH）后，口腔癌诱导4-硝基喹啉1-氧化物（4 NQO）。将28只雄性SD大鼠分为4组：第1组（未处理组）;第2组（对照组），仅饮用水中接受4种NQO 8周;第3组和第4组，饮用水中接受4种NQO 8周，并经口灌胃给予TH 1000 mg/kg和2000 mg/kg 10周。所有实验的所有大鼠在22周后处死，用显微镜评价口腔肿瘤的发生率和组织病理学变化。同时应用图像分析软件对舌标本进行免疫组化分析。通过使用RT 2 Profiler PCR Array（Qiagen，日耳曼敦，MD）评估与口腔癌相关的特定基因的表达。TH通过降低CCND 1、EGFR和考克斯-2的表达，显著降低口腔鳞状细胞癌（OSCC）的发病率，抑制癌细胞增殖。此外，TH通过过度表达β-catenin和e-cadherin维持细胞粘附（上皮极性），并通过下调TWIST 1和RAC 1抑制OSCC的侵袭性。我们的数据表明，TH发挥化学预防活性的动物模型中，口腔癌是由使用4 NQO诱导。
Objective. The aim of this study was to evaluate the chemopreventive activity of Malaysian jungle Tualang honey (TH) after oral carcinogenesis induced with 4-nitroquinoline 1-oxide (4 NQO).Study Design. A total of 28 male Sprague-Dawley (SD) rats were distributed into 4 groups as follows: group 1 (nontreated group); group 2 (control), which received 4 NQO during 8 weeks in drinking water only; and groups 3 and 4, which received 4 NQO for 8 weeks in drinking water and treated with TH 1000 mg/kg and 2000 mg/kg by oral gavage for 10 weeks. All rats from all experiments were sacrificed after 22 weeks, and the incidence of oral neoplasms and histopathologic changes were microscopically evaluated. Moreover, immunohistochemical expression was analyzed in tongue specimens by using image analysis software. The expression of particular genes associated with oral cancer were assessed by using RT2 Profiler PCR Array (Qiagen, Germantown, MD).Results. TH significantly reduced the incidence of oral squamous cell carcinoma (OSCC) and suppressed cancer cell proliferation via diminishing the expression of CCND1, EGFR, and COX-2. Furthermore, TH preserved cellular adhesion (epithelial polarity) through overexpression of beta-catenin and e-cadherin and inhibited the OSCC aggressiveness by downregulating TWIST1 and RAC1.Conclusions. Our data suggest that TH exerts chemopreventive activity in an animal model in which oral cancer was induced by using 4 NQO.