Collinear optical coherence and confocal fluorescence microscopies for tissue engineering
Collinear optical coherence and confocal fluorescence microscopies for tissue engineering
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DOI：
10.1364/oe.11.003074
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发表时间：
2003-11-17
期刊：
影响因子：
3.8
通讯作者：
Washburn, NR
中科院分区：
文献类型：
--
作者：
Dunkers, JP;Cicerone, MT;Washburn, NR
组织工程化医疗产品（TEMPs）通常是三维（3D）混合材料，由组织生长在其上的多孔支架组成。然而，监测支架内深处的发育组织受到其浊度的阻碍。我们一直在寻找新的方法来探测支架的内部，其分辨率与传统的激光扫描共聚焦显微镜相同，但具有更高的灵敏度。我们提出了一种新的应用光学相干显微镜（OCM），通过将其与共聚焦荧光显微镜（CFM），收集同步的结构和功能信息的TEMPs在一个注册的方式。在这项工作中，我们描述了共线OCM和CFM仪器。我们证明了这种双模式技术的实用性，通过成像荧光染色成骨细胞培养在聚合物TEMP。
Tissue engineered medical products (TEMPs) are often three-dimensional (3D) hybrid materials consisting of a porous scaffold upon which the tissue is grown. However, monitoring of the developing tissue deep within the scaffold is hampered by its turbidity. We have sought new ways to probe the interior of the scaffold with the same resolution as conventional laser scanning confocal microscopy but with greater sensitivity. We present a novel application of optical coherence microscopy (OCM) by combining it with confocal fluorescence microscopy (CFM) to gather simultaneous structural and functional information on TEMPs in a registered fashion. In this work, we describe the collinear OCM and CFM instrument. We demonstrate the utility of this dual-mode technique for TEMPs by imaging fluorescently stained osteoblasts cultured in a polymeric TEMP.