Carbon quantum dots derived from lysine and arginine simultaneously scavenge bacteria and promote tissue repair
Carbon quantum dots derived from lysine and arginine simultaneously scavenge bacteria and promote tissue repair
复制标题
来自赖氨酸和精氨酸的碳量子点同时清除细菌并促进组织修复
DOI：
10.1016/j.apmt.2020.100601
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发表时间：
2020-06-01
影响因子：
8.3
通讯作者：
Li, Bin
中科院分区：
文献类型：
--
作者：
Li, Peili;Han, Fengxuan;Li, Bin
有效地靶向和杀死微生物病原体是治疗感染性疾病的主要挑战。受天然抗菌肽（AMPs）高效性的启发，本研究通过裂解天然抗菌肽的两个主要功能部分赖氨酸和精氨酸制备了两种类型的碳量子点（CQD）。赖氨酸衍生的CQDs（Lys-CQDs）和精氨酸衍生的CQDs（Arg-CQDs）对革兰氏阴性菌和革兰氏阳性菌均表现出独特的抗菌活性，而不诱导耐药性。它们还有效地抑制了细菌生物膜的形成。这种优异的抗菌性能可能是由于CQD表面氨基酸残基所带的正电荷和细胞内丰富的活性氧（ROS）产生的结果。令人惊讶的是，CQD不仅没有细胞毒性，反而显著促进了典型哺乳动物细胞的生长。此外，当CQD浓度小于4，000 μ g/mL时，不发生红细胞（RBC）溶血。在体内，将CQDs施用到感染的伤口有效地抑制细菌生长并加速伤口愈合。总之，这项研究的结果表明，Lys-CQDs和Arg-CQDs可以同时杀死细菌并促进细胞生长，因此可能是治疗细菌感染和促进组织修复的有希望的候选者。(C)2020爱思唯尔有限公司保留所有权利。
Effectively targeting and killing microbial pathogens is a major challenge in the treatment of infectious diseases. Inspired by the high efficacy of natural antimicrobial peptides (AMPs), in this study we prepared two types of carbon quantum dots (CQDs) by pyrolysis of lysine and arginine, the two major functional moieties of natural AMPs. Both lysine-derived CQDs (Lys-CQDs) and arginine-derived CQDs (Arg-CQDs) exhibited distinctive antibacterial activity against both Gram-negative and Gram-positive bacteria without inducing drug resistance. They also effectively inhibited the formation of bacterial biofilms. Such excellent antibacterial properties might be the result of positive charges derived from the amino acid residues on CQD surface and the generation of rich intracellular reactive oxygen species (ROS). Surprisingly, instead of being cytotoxic, CQDs dramatically promoted the growth of typical mammalian cells. Moreover, no hemolysis of red blood cells (RBCs) happened when CQD concentration was less than 4,000 mu g/mL. In vivo , administration of CQDs to infected wounds effectively inhibited bacterium growth and accelerated wound healing. Together, findings from this study indicate that Lys-CQDs and Arg-CQDs can simultaneously kill bacteria and facilitate cell growth, and may therefore be promising candidates for treating bacterial infections and promoting tissue repair. (C) 2020 Elsevier Ltd. All rights reserved.