INTERACTION OF CELLS OF HELICOBACTER-PYLORI WITH HUMAN POLYMORPHONUCLEAR LEUKOCYTES - POSSIBLE ROLE OF HEMAGGLUTININS
INTERACTION OF CELLS OF HELICOBACTER-PYLORI WITH HUMAN POLYMORPHONUCLEAR LEUKOCYTES - POSSIBLE ROLE OF HEMAGGLUTININS
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DOI：
10.1111/j.1574-695x.1994.tb00472.x
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发表时间：
1994-06-01
影响因子：
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通讯作者：
WADSTROM, T
中科院分区：
文献类型：
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作者：
CHMIELA, M;LELWALAGURUGE, J;WADSTROM, T
研究了异硫氰酸荧光素(FITC)标记的幽门螺杆菌细胞与人多形核白细胞(PMN)的相互作用。为了确定幽门螺杆菌细胞表面血凝素是否在凝集素介导的与吞噬细胞的结合/摄取中发挥作用，我们使用了两个具有不同受体特异性的表面血凝素的菌株：(1)表达唾液酸特异性血凝素的菌株17874(NCTC 11637)和(2)表达唾液酸非依赖性血凝素的菌株17875(NCTC 11638)。与菌株17875的细胞相比，菌株17874的细胞在PMN上的附着/摄取较差。用胎球蛋白或兔抗部分纯化的唾液酸特异性血凝素的抗体预处理细菌可增强17874株细胞与中性粒细胞的相互作用。在菌株17875的情况下，这种增强没有发生。抗幽门螺杆菌抗体阳性的新鲜人血清对幽门螺杆菌17874细菌的吞噬功能有轻微的促进作用。结果表明，17874种细菌的唾液酸特异性血凝素复合体可能干扰了人中性粒细胞对它们的摄取。
The interaction of fluorescein isothiocyanate (FITC)-labelled cells of Helicobacter pylori with human polymorphonuclear leucocytes (PMNs) was studied. Two strains with surface haemagglutinins expressing different receptor specificity were used in order to decide if cell surface haemagglutinins of H. pylori may play a role in lectin-mediated binding to/uptake by phagocytes: (1) strain 17874 (NCTC 11637) which expresses sialic acid-specific haemagglutinin; and (2) strain 17875 (NCTC 11638) which expresses a sialic acid-independent haemagglutinin. Cells of strain 17874 were poorly attached to/ingested by PMNs compared to cells of strain 17875. Pre-treatment of bacteria with fetuin or rabbit antibodies against partly purified sialic acid-specific haemagglutinin enhanced interaction of cells of strain 17874 with PMNs. The enhancement did not occur in the case of strain 17875. Phagocytosis of H. pylori 17874 bacteria was slightly increased by fresh human sera positive for anti-II pylori antibodies. The results suggest that the sialic acid-specific haemagglutinin complex of 17874 bacteria might disturb their uptake by human PMNs.