A Novel Experimental Animal Model of Arterial Stenosis Based on Endovascular Radiofrequency Energy Application
A Novel Experimental Animal Model of Arterial Stenosis Based on Endovascular Radiofrequency Energy Application
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DOI：
10.3109/08941939.2011.557470
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发表时间：
2011-04-01
影响因子：
1.9
通讯作者：
Habib, Nagy
中科院分区：
文献类型：
--
作者：
Lazoura, Olga;Zacharoulis, Dimitris;Habib, Nagy
目的：利用血管内射频（RF）能量开发一种新的兔动脉狭窄模型。材料和方法：10 只兔子通过 Habib (TM)(TM) VesCoag (TM)(TM) 导管对主动脉和左颈总动脉进行多次血管内射频应用。手术后立即和六周后使用血管造影和彩色多普勒超声评估血管通畅性。在进行血管壁组织病理学分析的程序后处死一只兔子。两只兔子在手术后立即分别死于主动脉和颈动脉破裂。其余七只兔子在六周随访后被处死进行组织病理学分析。结果：诱导显着动脉狭窄（> 50%%）且无并发症的最佳射频发生器设置，主动脉标准化为 24--26 瓦 (W) 1.5 分钟，颈总动脉标准化为 6 W 1 分钟。六周的随访显示所有幸存的兔子都得到了永久性的结果。组织病理学显示内膜和内侧平滑肌层坏死。结论：我们利用血管内射频能量开发了一种新型兔动脉狭窄模型。我们的模型快速、安全、廉价且可重复。它将对于实验研究和新的治疗设备有用。
Purpose: To develop a new rabbit model of arterial stenosis using endovascular radiofrequency (RF) energy. Materials and Methods: Ten rabbits were used for multiple endovascular RF applications to the aorta and left common carotid artery through the Habib (TM)(TM) VesCoag (TM)(TM) catheter. Angiography and color Doppler ultrasound were used to assess vessel patency immediately following the procedure and six weeks later. One rabbit was sacrificed following the procedure for histopathologic analysis of the vessel wall. Two rabbits died of aortic and carotid rupture, respectively, immediately after the procedure. The remaining seven rabbits were sacrificed after six-week follow-up for histopathological analysis. Results: Optimal RF generator settings to induce significant arterial stenosis (> 50%%) without complications were standardized at 24--26 watts (W) for 1.5 min for the aorta and 6 W for 1 min for the common carotid artery. The six-week follow-up showed permanent results in all surviving rabbits. Histopathology revealed intima and medial smooth muscle layer necrosis. Conclusion: We have developed a novel rabbit model of arterial stenosis using endovascular RF energy. Our model is fast, safe, inexpensive, and reproducible. It would be useful for experimental investigations and new therapeutic devices.