A further link between innate and adaptive immunity:: C3 deposition on antigen-presenting cells enhances the proliferation of antigen-specific T cells
A further link between innate and adaptive immunity:: C3 deposition on antigen-presenting cells enhances the proliferation of antigen-specific T cells
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DOI：
10.1093/intimm/10.12.1923
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发表时间：
1998-12-01
影响因子：
4.4
通讯作者：
Erdei, A
中科院分区：
文献类型：
--
作者：
Kerekes, K;Prechl, J;Erdei, A
类淋巴母细胞系 A20 和伴刀豆球蛋白 A 诱导的腹膜巨噬细胞的鼠细胞在新鲜自体血清中在允许启动替代补体途径的条件下孵育时，可共价激活和固定 C3 片段。为了检测细胞结合的C3，使用FITC标记的抗小鼠C3的F(ab')(2)片段进行细胞荧光测定，在培养条件下至少两个小时，细胞结合的C3片段不会被细胞内化或脱落，当使用各种抗原和实验系统测试血清处理的细胞的抗原呈递能力时，发现抗原特异性T细胞的增殖增强，这种增强效果在次优抗原时尤其明显剂量。 C3 调理抗原呈递细胞 (APC) 诱导的 T 细胞增殖升高可以被山羊抗小鼠 C3 的 F(ab')(2) 片段消除，这表明 T 细胞表达的 C3 受体参与了这一过程。使用识别鼠 CR1/CR2 的 7G6 mAb，通过细胞荧光测定和免疫沉淀法证明了这些补体受体在活化的 T 细胞上的存在。这些结果表明了C3 与 APC 上的受体位点结合，促进抗原呈递，在先天免疫和适应性免疫之间提供了进一步的联系。
Murine cells of the a lymphoblastoid line A20 and concanavalin A-elicited peritoneal macrophages are shown to activate and fix C3 fragments covalently when incubated in fresh, autologous serum under conditions allowing the initiation of the alternative complement pathway. For the detection of cell-bound C3, cytofluorimetry was performed using FITC-labeled F(ab')(2) fragments of anti-mouse C3, Cell-bound C3 fragments are not internalized or shed by the cells under culture conditions for at least two hours, When the antigen-presenting capacity of serum-treated cells was tested using various antigens and experimental systems, augmentation of the proliferation of antigen-specific T cells was found, This enhancing effect was particularly pronounced at suboptimal antigen doses. The elevation of T cell proliferation induced by C3-opsonized antigen-presenting cells (APC) could be abrogated by F(ab')(2) fragments of goat anti-mouse C3, suggesting the involvement of C3 receptors expressed by T cells in the process, Using the 7G6 mAb recognizing murine CR1/CR2, the presence of these complement receptors on activated T cells is demonstrated by cytofluorimetry and immunoprecipitation, as well, These results point to the role of C3 bound to acceptor sites on APC in the facilitation of antigen presentation, providing a further link between innate and adaptive immunity.