Effect of binary combinations of selected toxic compounds on growth and fermentation of Kluyveromyces marxianus
Effect of binary combinations of selected toxic compounds on growth and fermentation of Kluyveromyces marxianus
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DOI：
10.1021/bp034317p
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发表时间：
2004-05-01
影响因子：
2.9
通讯作者：
Ballesteros, M
中科院分区：
文献类型：
--
作者：
Oliva, JM;Ballesteros, I;Ballesteros, M
在分批培养中研究了各种木质纤维素降解产物对耐热酵母马克斯克鲁维酵母葡萄糖发酵的抑制作用。研究了芳香醇儿茶酚和两种芳香醛（4-羟基苯甲醛和香草醛）的二元组合的毒性。还测试了戊糖降解的水解产物中通常以相对高浓度存在的醛糠醛。实验是通过将不同浓度的药物组合起来进行的，单独使用时会抑制 25% 的生长。与单个化合物的相对毒性相比，糠醛与儿茶酚和4-羟基苯甲醛的组合具有累加性（生长抑制50%）。所测定的其他二元组合（儿茶酚与 4-羟基苯甲醛，以及香草醛与儿茶酚、糠醛或 4-羟基苯甲醛）显示出对毒性的协同作用，并导致细胞产量减少 60-90%。发酵培养基中醛的存在强烈抑制细胞生长和乙醇生产。马克斯克鲁维酵母将醛还原为其相应的醇，以减轻这些化合物的毒性。需要完全减少醛才能开始生产乙醇。香草醛在二元组合中具有剧毒，并且是唯一无法通过酵母菌株同化克服抑制作用的化合物，导致细胞生长和发酵减少 90%。
The inhibitory effects of various lignocellulose degradation products on glucose fermentation by the thermotolerant yeast Kluyveromyces marxianus were studied in batch cultures. The toxicity of the aromatic alcohol catechol and two aromatic aldehydes (4-hydroxybenzaldehyde and vanillin) was investigated in binary combinations. The aldehyde furfural that usually is present in relatively high concentration in hydrolyzates from pentose degradation was also tested. Experiments were conducted by combining agents at concentrations that individually caused 25% inhibition of growth. Compared to the relative toxicity of the individual compounds, combinations of furfural with catechol and 4-hydroxybenzaldehyde were additive (50% inhibition of growth). The other binary combinations assayed (catechol with 4-hydroxybenzaldehyde, and vanillin with catechol, furfural, or 4-hydroxybenzaldehyde) showed synergistic effect on toxicity and caused a 60-90% decrease in cell mass production. The presence of aldehydes in the fermentation medium strongly inhibited cell growth and ethanol production. Kluyveromyces marxianus reduces aldehydes to their corresponding alcohols to mitigate the toxicity of these compounds. The total reduction of aldehydes was needed to start ethanol production. Vanillin, in binary combination, was dramatically toxic and was the only compound for which inhibition could not be overcome by yeast strain assimilation, causing a 90% reduction in both cell growth and fermentation.