Genetic dissection of a mammalian replicator in the human β-globin locus
Genetic dissection of a mammalian replicator in the human β-globin locus
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DOI：
10.1126/science.281.5379.1005
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发表时间：
1998-08-14
期刊：
影响因子：
56.9
通讯作者：
Wahl, GM
中科院分区：
文献类型：
--
作者：
Aladjem, MI;Rodewald, LW;Wahl, GM
哺乳动物细胞中DNA复制起始的时间和位置是可遗传的特征，但尚不清楚起始是否需要特定的DNA序列。使用位点特异性重组策略来显示先前鉴定为复制起始位点的DNA序列在转移到新的染色体位置时可以启动复制。包含人β-珠蛋白基因座中DNA复制起点的8-羟色胺酶DNA序列启动了猿基因组中的复制。珠蛋白起始点内的特异性缺失不会启动这些染色体位点的复制。这些数据表明，在哺乳动物细胞中的DNA复制的启动需要特定的序列信息，并扩展复制子假说高等真核生物。
The timing and localization of DNA replication initiation in mammalian cells are heritable traits, but it is not known whether initiation requires specific DNA sequences. A site-specific recombination strategy was used to show that DNA sequences previously identified as replication initiation sites could initiate replication when transferred to new chromosomal Locations. An 8-kilobase DNA sequence encompassing the origin of DNA replication in the human P-globin Locus initiated replication in the simian genome. Specific deletions within the globin origin did not initiate replication in these chromosomal sites. These data suggest that initiation of DNA replication in mammalian cells requires specific sequence information and extend the replicon hypothesis to higher eukaryotes.