TIMP-3 is expressed in the human retinal pigment epithelium.
TIMP-3 is expressed in the human retinal pigment epithelium.
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TIMP-3 在人视网膜色素上皮细胞中表达。
DOI：
10.1006/bbrc.1996.1379
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Bok,D
中科院分区：
文献类型：
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作者：
Ruiz,A;Brett,P;Bok,D
TIMP-3是金属蛋白酶组织抑制剂（TIMP）家族的最新成员。在本研究中，我们描述了TIMP-3信使RNA（mRNA）的表达由视网膜色素上皮细胞的正常人眼（hRPE）。除了在成人和胎儿阶段的几种其他人体组织中发现的约5.1、2.8和2.4 Kbp的三种主要转录物之外。hRPE还表达1.2和1.0 Kbp的两种RNA种类。基于从hRPE cDNA文库中分离的cDNA克隆的序列分析，使用交替的多聚腺苷酸化信号可以解释这些较小的转录本的表达。不排除RPE调节TIMP-3表达的替代机制的可能性。通过RT-PCR定量每纳克聚A+RNA对TIMP-3特异的RNA转录物的数目。在成体阶段每纳克polyA+RNA可检测到9.6× 105个转录本，在胎儿阶段每纳克polyA+RNA可检测到1.2× 106个转录本。这些发现得到了视网膜组织切片中RPE层原位杂交实验的优势标记的支持。所有这些数据都支持这样的假设，即RPE产生TIMP-3可能对维持布鲁赫膜至关重要，布鲁赫膜是细胞外基质的复杂层，其为健康视网膜中的RPE提供结构基质，并且在衰老过程中以及在显性遗传性疾病Sorby眼底营养不良中受到干扰。
TIMP-3 is the most recent member of the tissue inhibitor of metalloproteinases (TIMP) family. In the present study, we describe the expression of TIMP-3 messenger RNA (mRNA) by the retinal pigment epithelium of the normal human eye (hRPE). In addition to the three predominant transcripts of approximately 5.1, 2.8, and 2.4 Kbp found in several other human tissues at adult and fetal stages. The hRPE also expresses two RNA species of 1.2 and 1.0 Kbp. Based on the sequence analysis of cDNA clones isolated from a hRPE cDNA library, the use of alternate polyadenylation signals could account for the expression of these smaller transcripts. The possibility of an alternative mechanism of regulation of the expression of TIMP-3 by the RPE is not discarded. The number of RNA transcripts specific for TIMP-3 per nanogram of poly A+RNA was quantified by RT-PCR. 9.6×105transcripts per nanogram of polyA+RNA were found at the adult stage and 1.2×106transcripts per nanogram of polyA+RNA were detected at the fetal stage. These findings were supported by the predominant labeling in the RPE layer of retinal tissue sections inin situhybridization experiments. All of these data support the hypothesis that the production of TIMP-3 by the RPE may be crucial for the maintenance of Bruch's membrane, the complex layer of extracellular matrix that provides a structural substrate for the RPE in the healthy retina and is perturbed during the ageing process and in Sorby's Fundus Dystrophy, a dominantly inherited disease.