Staphylococcus aureus genetic loci impacting growth and survival in multiple infection environments
Staphylococcus aureus genetic loci impacting growth and survival in multiple infection environments
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DOI：
10.1046/j.1365-2958.1998.01075.x
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发表时间：
1998-10-01
影响因子：
3.6
通讯作者：
Stover, CK
中科院分区：
文献类型：
--
作者：
Coulter, SN;Schwan, WR;Stover, CK
革兰氏阳性细菌金黄色葡萄球菌感染不同的组织，并导致广泛的疾病，这表明它具有不同的分子机制，促进细菌在不同的体内环境中的生存。通过对1520个成员的文库进行信号标签转座子诱变筛选，鉴定出大量的S。金黄色葡萄球菌基因位点影响生长和生存的四个互补的动物感染模型，包括小鼠脓肿，菌血症和伤口和兔心内膜炎。在通过鼠模型鉴定的总共237种体内减毒突变体中，在所有三种模型中不到10%显示减毒，强调了在不同疾病环境中筛选的优势。通过这些分析确定的最大基因类别编码肽和氨基酸转运蛋白，其中一些对S。金黄色葡萄球菌在所有测试的动物感染模型中存活。鉴定葡萄球菌在多种组织环境中影响生长、持久性和毒力的基因座，有助于深入了解人类感染的复杂性和新的抗菌疗法可能靶向的分子机制。
The Gram-positive bacterium Staphylococcus aureus infects diverse tissues and causes a wide spectrum of diseases, suggesting that it possesses a repertoire of distinct molecular mechanisms promoting bacterial survival in disparate in vivo environments. Signature-tag transposon mutagenesis screening of a 1520-member library identified numerous S. aureus genetic loci affecting growth and survival in four complementary animal infection models including mouse abscess, bacteraemia and wound and rabbit endocarditis. Of a total of 237 in vivo attenuated mutants identified by the murine models, less than 10% showed attenuation in all three models, emphasizing the advantage of screening in diverse disease environments. The largest gene class identified by these analyses encoded peptide and amino acid transporters, some of which were important for S. aureus survival in all animal infection models tested. The identification of staphylococcal loci affecting growth, persistence and virulence in multiple tissue environments provides insight into the complexities of human infection and on the molecular mechanisms that could be targeted by new antibacterial therapies.