A synoviocyte model for osteoarthritis and rheumatoid arthritis: response to Ibuprofen, betamethasone, and ginger extract-a cross-sectional in vitro study.
A synoviocyte model for osteoarthritis and rheumatoid arthritis: response to Ibuprofen, betamethasone, and ginger extract-a cross-sectional in vitro study.
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用于骨关节炎和类风湿关节炎的滑膜细胞模型：对布洛芬，倍他倍疑和姜提取物的反应 - A横截面体外研究。
DOI：
10.1155/2012/505842
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Bliddal H
中科院分区：
文献类型：
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作者：
Ribel-Madsen S;Bartels EM;Stockmarr A;Borgwardt A;Cornett C;Danneskiold-Samsøe B;Bliddal H
本研究旨在确定类风湿关节炎(RA)、骨性关节炎(OA)和健康对照(HC)的滑膜细胞培养是否不同，是否适合用于药理学研究中的疾病模型，并测试它们对一些抗炎药物的反应。从滑膜或关节液中分离滑膜细胞。在没有倍他米松、布洛芬或标准化生姜提取物存在的情况下，培养细胞并暴露在NO或肿瘤坏死因子-α刺激下。一组细胞因子、生长因子和趋化因子的浓度被映射到每种培养和条件下。我们的细胞在所有条件下都能分泌更多的细胞因子IL-1β、IL-6和IL-8，以响应肿瘤坏死因子-α的刺激。未受刺激时，OA细胞的IL-6和IL-8水平高于RA和HC细胞，而IL-1β的产生水平低于RA和HC细胞。肿瘤坏死因子-α刺激引起各组IL-1β、IL-6和IL-8的释放相似。布洛芬对细胞因子的产生没有影响，而生姜提取物与倍他米松相似。在此体外模型中，生姜提取物的抗炎作用与倍他米松相同。从OA和RA受试者培养的成纤维细胞样滑膜细胞有望成为一种有用的药理学疾病模型，但还需要进一步的研究来支持这种模型的结果。
This study aimed at determining if synovial cell cultures from rheumatoid arthritis (RA), osteoarthritis (OA), and healthy controls (HC) differ and are suitable disease models in pharmacological studies, and tested their response to some anti-inflammatory drugs. Synovial cells were isolated from synovial membrane or joint fluid. Cells were cultivated and exposed to no or TNF-α stimulation without, or in the presence of, betamethasone, ibuprofen, or a standardized ginger extract. Concentrations of a panel of cytokines, growth factors, and chemokines were mapped for each culture and condition. Our cells secreted an increased amount of the cytokines IL-1β, IL-6, and IL-8 in response to TNF-α stimulation in all conditions. OA cells showed a higher IL-6 and IL-8 and a lower IL-1β production, when not stimulated, than RA and HC cells, which were similar. TNF-α stimulation caused similar IL-1β, IL-6, and IL-8 release in all groups. Ibuprofen showed no effect on cytokine production, while ginger extract was similar to betamethasone. Ginger extract was as effective an anti-inflammatory agent as betamethasone in this in vitro model. Cultured fibroblast-like synoviocytes from OA and RA subjects promise to be a useful pharmacological disease model, but further studies, to support results from such a model are needed.