Detection of tumour lymphovascular space invasion using dual cytokeratin and CD31 immunohistochemistry
Detection of tumour lymphovascular space invasion using dual cytokeratin and CD31 immunohistochemistry
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DOI：
10.1136/jcp.56.10.786
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发表时间：
2003-10-01
影响因子：
3.4
通讯作者：
Jacobs, IJ
中科院分区：
文献类型：
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作者：
Alexander-Sefre, F;Singh, N;Jacobs, IJ
背景：血管间隙浸润（LVSI）是肿瘤转移复杂过程中的重要一步。各种方法已被用于在过去，以提高LVSI.Aims的组织学检测：建立一个敏感的免疫组化方法检测LVSI.Methods：石蜡块从108例患者行子宫切除术的I期子宫内膜癌。从这些组织块切下的4 μ m切片上进行泛细胞角蛋白和CD31内皮细胞标记物的双重免疫染色，并与常规苏木精和伊红染色进行比较。这一发现表明LVSI是一种比以前认为的更常见的现象，并质疑目前对肿瘤转移的理解。
Background: Lymphovascular space invasion (LVSI) is an important step in the complex process of tumour metastasis. Various methods have been used in the past to improve the histological detection of LVSI.Aims: To develop a sensitive immunohistochemical method for the detection of LVSI.Methods: Paraffin wax blocks from 108 patients who had undergone hysterectomy for stage I endometrial cancer were retrieved. Dual immunostaining for pancytokeratin and the CD31 endothelial cell marker was carried out on 4 mum sections cut from these bocks and compared with conventional haematoxylin and eosin staining.Results: The detection rate for LVSI increased threefold compared with conventional haematoxylin and eosin staining in the test group.Conclusion: This finding suggests that LVSI is a much more common phenomenon than previously thought and questions current understanding of tumour metastasis.