Delivery of short hairpin RNA sequences by using a replication-competent avian retroviral vector
Delivery of short hairpin RNA sequences by using a replication-competent avian retroviral vector
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DOI：
10.1128/jvi.78.9.4914-4916.2004
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发表时间：
2004-05-01
影响因子：
5.4
通讯作者：
Holmen, SL
中科院分区：
文献类型：
--
作者：
Bromberg-White, JL;Webb, CP;Holmen, SL
虽然最近的研究已经证明逆转录病毒载体可以用于稳定表达短发夹RNA（shRNA）以抑制基因表达，但是这些研究利用了复制缺陷型逆转录病毒。我们描述了一个复制能力，网关兼容的逆转录病毒载体，能够表达抑制特定基因表达的shRNA的创建。
While recent studies have demonstrated that retroviral vectors can be used to stably express short hairpin RNA (shRNA) to inhibit gene expression, these studies have utilized replication-defective retroviruses. We describe the creation of a replication-competent, Gateway-compatible retroviral vector capable of expressing shRNA that inhibits the expression of specific genes.