Expression profile of PIN, AUX/LAX and PGP auxin transporter gene families in Sorghum bicolor under phytohormone and abiotic stress
Expression profile of PIN, AUX/LAX and PGP auxin transporter gene families in Sorghum bicolor under phytohormone and abiotic stress
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DOI：
10.1111/j.1742-4658.2010.07706.x
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发表时间：
2010-07
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通讯作者：
Chenjia Shen;Youhuang Bai;Suikang Wang;Saina Zhang;Yunrong Wu;Ming Chen;D. Jiang;Yanhua Qi
中科院分区：
文献类型：
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作者：
Chenjia Shen;Youhuang Bai;Suikang Wang;Saina Zhang;Yunrong Wu;Ming Chen;D. Jiang;Yanhua Qi
生长素由内流载体生长素抗性1/类似物aux 1（AUX/LAX）和外流载体pin‐formed（PIN）和P‐glycoprotein（PGP）转运，它们在生长素极性转运中起主要作用。一些生长素转运蛋白基因已在双子叶植物拟南芥中被鉴定，但大多数在单子叶植物，特别是高粱中是未知的。本文分析了SbPIN、SbLAX和SbPGP基因家族在拟南芥、水稻和高粱中的染色体分布、基因重复和内含子/外显子，并研究了它们在拟南芥、水稻和高粱中的遗传关系。真实的荧光定量PCR分析表明，这些基因中的大多数在高粱的器官中有不同的表达。SbPIN 3和SbPIN 9在花中高表达，SbLAX 2和SbPGP 17主要在茎中表达，SbPGP 7在根中强表达。这表明单个基因可能参与特定器官的发育。这些基因家族的表达谱进行了分析处理后：（a）植物激素吲哚-3-乙酸和油菜素类固醇;（B）极性生长素运输抑制剂1-萘氧基乙酸，1-萘基邻苯二甲酰胺酸和2，3，5-三碘苯甲酸;和（c）脱落酸和高盐和干旱的非生物胁迫。大多数生长素转运蛋白基因都受到吲哚-3-乙酸和油菜素类固醇的强烈诱导，为这些植物激素的协同作用提供了新的证据。有趣的是，大多数基因在极性生长素运输抑制剂下表现出相似的表达趋势，并且每个基因也对脱落酸、盐和干旱有反应。本研究为高粱生长素转运蛋白的研究提供了新的思路。
Auxin is transported by the influx carriers auxin resistant 1/like aux1 (AUX/LAX), and the efflux carriers pin‐formed (PIN) and P‐glycoprotein (PGP), which play a major role in polar auxin transport. Several auxin transporter genes have been characterized in dicotyledonous Arabidopsis, but most are unknown in monocotyledons, especially in sorghum. Here, we analyze the chromosome distribution, gene duplication and intron/exon of SbPIN, SbLAX and SbPGP gene families, and examine their phylogenic relationships in Arabidopsis, rice and sorghum. Real‐time PCR analysis demonstrated that most of these genes were differently expressed in the organs of sorghum. SbPIN3 and SbPIN9 were highly expressed in flowers, SbLAX2 and SbPGP17 were mainly expressed in stems, and SbPGP7 was strongly expressed in roots. This suggests that individual genes might participate in specific organ development. The expression profiles of these gene families were analyzed after treatment with: (a) the phytohormones indole‐3‐acetic acid and brassinosteroid; (b) the polar auxin transport inhibitors 1‐naphthoxyacetic acids, 1‐naphthylphthalamic acid and 2,3,5‐triiodobenzoic acid; and (c) abscissic acid and the abiotic stresses of high salinity and drought. Most of the auxin transporter genes were strongly induced by indole‐3‐acetic acid and brassinosteroid, providing new evidence for the synergism of these phytohormones. Interestingly, most genes showed similar trends in expression under polar auxin transport inhibitors and each also responded to abscissic acid, salt and drought. This study provides new insights into the auxin transporters of sorghum.