Nucleotide sequence and expression of a novel human receptor-type tyrosine kinase gene (flt) closely related to the fms family.
Nucleotide sequence and expression of a novel human receptor-type tyrosine kinase gene (flt) closely related to the fms family.
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发表时间：
1990-04
期刊：
影响因子：
8
通讯作者：
M. Shibuya;S. Yamaguchi;A. Yamane;T. Ikeda;A. Tojo;H. Matsushime;Misako Sato
中科院分区：
文献类型：
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作者：
M. Shibuya;S. Yamaguchi;A. Yamane;T. Ikeda;A. Tojo;H. Matsushime;Misako Sato
通过与v-ros癌基因弱交叉杂交，分离到一个新的受体型酪氨酸激酶基因。全长约7.7 kb的cDNA携带一个4.2 kb的开放阅读框，预测的1338个残基氨基酸序列包含胞外、跨膜和酪氨酸激酶结构域。虽然它的胞外结构域比fms家族的产物，即c-fms、c-kit和血小板衍生的生长因子受体基因长约220个氨基酸，但其胞外结构域的整体结构包括半胱氨酸基元和酪氨酸激酶结构域的长肽插入，表明该新基因与fms家族密切相关。因此，该基因被命名为flt （fms-like tyrosine kinase）基因。flt基因的表达在大多数肿瘤细胞系中被强烈抑制，而该mRNA在成年大鼠的多种正常组织中均有表达。
A new human gene encoding a receptor-type tyrosine kinase was isolated by a weak cross-hybridization with v-ros oncogene. A cDNA of about 7.7 kb carried a 4.2 kb open reading frame, and the predicted amino acid sequence of 1338 residues contained extracellular, transmembrane and tyrosine kinase domains. Although its extracellular domain is approximately 220 amino acids longer than those of the products of the fms family, i.e. c-fms, c-kit and platelet-derived growth factor receptor genes, the overall structure including cysteine motifs in its extracellular domain and a long peptide insertion in its tyrosine kinase domain indicates that this new gene is closely related to the fms family. Consequently, the gene was designated as flt (fms-like tyrosine kinase) gene. The expression of the flt gene was strongly suppressed in most of the tumor cell lines examined so far, whereas this mRNA was expressed in a variety of normal tissues of adult rat.