Molecular cloning of BNP from heart and its immunohistochemical localization in the hypothalamus of monkey
Molecular cloning of BNP from heart and its immunohistochemical localization in the hypothalamus of monkey
复制标题
DOI：
10.1016/j.peptides.2006.01.001
复制
发表时间：
2006-07-01
期刊：
影响因子：
3
通讯作者：
Tooyama, Ikuo
中科院分区：
文献类型：
--
作者：
Abdelalim, Essam Mohamed;Takada, Tatsuyuki;Tooyama, Ikuo
以往的生理学研究表明，脑钠肽（BNP）的核心作用。然而，关于BNP在大脑中的定位的信息很少。在这项研究中，我们确定了编码日本和食蟹猴的前BNP原的整个编码区的cDNA序列，然后研究了BNP在猴下丘脑的免疫组织化学定位。日本猴和食蟹猴前BNP原由132个氨基酸残基组成，具有生物活性的C-末端32个氨基酸。不同物种间推导的氨基酸序列的比较显示猴和人之间具有较高的同源性（prerpro-BNP为91%，成熟区为97%）。免疫组化结果显示，猴下丘脑室旁核、室周核和视上核内均可见BNP免疫反应阳性斑点。提示BNP在下丘脑神经内分泌系统中具有中枢作用。(c)2006年爱思唯尔公司All rights reserved.
Previous physiological studies have suggested central roles of brain natriuretic peptide (BNP). However, little information is available about the localization of BNP in the brain. In this study, we determined cDNA sequence encoding the entire coding region of prepro-BNP of Japanese and cynomologus monkeys, and then examined the immunohistochemical localization of BNP in the monkey hypothalamus. Japanese and cynomologus monkey prepro-BNP consisted of 132 amino acid residues with biologically active C-terminal 32 amino acids. Comparisons of deduced amino acid sequences among different species revealed high homology between monkey and human (91% in prerpro-BNP and 97% in the mature region). Immunohistochemical examination showed that BNP immunoreactive dots were observed in the paraventricular, periventricular, and supraoptic nuclei of the monkey hypothalamus. The present result suggests the central role of BNP in the neuroendocrine system in the hypothalamus. (c) 2006 Elsevier Inc. All rights reserved.