A new confocal stigmatic spectrometer for micro-Raman and microfluorescence spectral imaging analysis: Design and applications
A new confocal stigmatic spectrometer for micro-Raman and microfluorescence spectral imaging analysis: Design and applications
复制标题
用于微拉曼和微荧光光谱成像分析的新型共焦象散光谱仪：设计与应用
DOI：
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
M. Manfait
中科院分区：
文献类型：
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作者：
A. Feofanov;S. Sharonov;P. Valisa;E. D. Silva;I. Nabiev;M. Manfait
研制了一种新的共焦光谱成像仪，其横向分辨率为1.03 μm，轴向分辨率为1.0 μm，样品面积为5×5 ~ 150×150 μm。模块化拉曼/荧光光谱仪配备有CCD相机、显微镜、电动样品台和共焦入口室。一个由检流计控制的镜扫描仪系统配备了共焦入口室，允许光谱平行积累到数百个样品点，具有可调的光谱和空间分辨率。拉曼光谱仪的杂散光抑制特性提供了在低频域记录拉曼光谱图像的可能性。开发了一种软件来控制图像积累、创建和处理。应用于多维光谱集的光谱分析方法允许创建多种形式的光谱图像。为了创建这些图像，不同的光谱p...
A new instrument was developed permitting confocal spectral imaging technique to be carried out with a lateral resolution ∼0.3 μm and an axial resolution ∼1.0 μm for specimen areas ranging from 5×5 to 150×150 μm. The modular Raman/fluorescence spectrometer was equipped with a CCD camera, microscope, motorized sample stage, and the confocal entrance chamber. A system of galvanometer controlled mirror scanners equipped the confocal entrance chamber, allowing spectra to be accumulated up to several hundreds of points of sample in parallel, with adjustable spectral and spatial resolution. Stray light rejection property of the Raman spectrometer provides the possibility of the Raman spectral image to be recorded in the low frequency domain. A software was developed to control image accumulation, creation, and treatment. The methods of spectral analysis being applied to a multidimensional set of spectra permit the multiform spectral images to be created. In order to create these images, the different spectral p...