Morphogenes bolA and mreB mediate the photoregulation of cellular morphology during complementary chromatic acclimation in Fremyella diplosiphon
Morphogenes bolA and mreB mediate the photoregulation of cellular morphology during complementary chromatic acclimation in Fremyella diplosiphon
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DOI：
10.1111/mmi.12649
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发表时间：
2014-07-01
影响因子：
3.6
通讯作者：
Montgomery, Beronda L.
中科院分区：
文献类型：
--
作者：
Singh, Shailendra P.;Montgomery, Beronda L.
互补色驯化（CCA）过程中的色素沉着的光调节在Fremyella diplosiphon中得到了很好的研究，然而，CCA相关的形态变化的机制见解仍然是新兴的。F.在绿色光（GL）下，双虹吸管细胞为矩形，而在红光（RL）下，细胞较小且为球形。在这里，我们调查的作用，形态基因bolA和mreB在CCA使用基因表达和基因功能分析。食品药品管理diplosiphon bolA基因是必需的，因为它从基因组中完全去除是不成功的。消耗bolA导致生长缓慢，形态缺陷和高水平的活性氧物种的积累在一个部分隔离的三角洲bolA菌株。在RL下观察到bolA的较高表达，并且与野生型中mreB和mreC基因的较低表达相关。在缺乏红/绿响应性RcaE光感受器的Delta rcaE菌株中，bolA和mre基因的表达在RL和GL下均发生改变。观察到的基因表达关系表明，mreB和mreC的表达受到bolA表达的RcaE依赖性光调节的控制。表达F.在大肠杆菌中的diplosiphon bolA和mreB同源物证明了所编码蛋白的功能保守性。总之，这些研究确立了bolA和mreB在RcaE依赖的细胞形态调节中的作用。
Photoregulation of pigmentation during complementary chromatic acclimation (CCA) is well studied in Fremyella diplosiphon; however, mechanistic insights into the CCA-associated morphological changes are still emerging. F. diplosiphon cells are rectangular under green light (GL), whereas cells are smaller and spherical under red light (RL). Here, we investigate the role of morphogenes bolA and mreB during CCA using gene expression and gene function analyses. The F. diplosiphon bolA gene is essential as its complete removal from the genome was unsuccessful. Depletion of bolA resulted in slow growth, morphological defects and the accumulation of high levels of reactive oxygen species in a partially segregated Delta bolA strain. Higher expression of bolA was observed under RL and was correlated with lower expression of mreB and mreC genes in wild type. In a Delta rcaE strain that lacks the red-/green-responsive RcaE photoreceptor, the expression of bolA and mre genes was altered under both RL and GL. Observed gene expression relationships suggest that mreB and mreC expression is controlled by RcaE-dependent photoregulation of bolA expression. Expression of F. diplosiphon bolA and mreB homologues in Escherichia coli demonstrated functional conservation of the encoded proteins. Together, these studies establish roles for bolA and mreB in RcaE-dependent regulation of cellular morphology.