Fabrication of gradient hydrogels using a microfluidics/photopolymerization process
Fabrication of gradient hydrogels using a microfluidics/photopolymerization process
复制标题
DOI：
10.1021/la049298n
复制
发表时间：
2004-06-22
期刊：
影响因子：
3.9
通讯作者：
Langer, R
中科院分区：
文献类型：
--
作者：
Burdick, JA;Khademhosseini, A;Langer, R
介绍了一种制备具有固定分子和交联密度梯度的光交联水凝胶的方法。将两种大分子单体/引发剂溶液注入独特的聚（二甲基硅氧烷）通道系统中，产生预聚物梯度，随后在紫外线照射下聚合成水溶胀的水凝胶。梯度由进样流速控制（优化为每个入口 0.3 μL/min 以产生线性梯度）。该技术通过制造调节附着内皮细胞的空间分布的粘附配体梯度和导致独特的水凝胶结构和空间依赖性膨胀的交联密度梯度来研究。
A method of fabricating photo-cross-linked hydrogels with gradients of immobilized molecules and cross-linking densities is introduced. Two macromer/initiator solutions are injected into a unique poly(dimethylsiloxane) channel system that produces a prepolymer gradient that is subsequently polymerized into a water-swollen hydrogel with ultraviolet light exposure. The gradient is controlled by the injection flow rate (optimized to 0.3 muL/min per inlet to produce a linear gradient). The technique is investigated both through fabrication of adhesive ligand gradients that modulate spatial distribution of attached endothelial cells and gradients of cross-linking densities that led to unique hydrogel architectures and spatially dependent swelling.