Baculovirus infection blocks the progression of fat body degradation during metamorphosis in Bombyx mori
Baculovirus infection blocks the progression of fat body degradation during metamorphosis in Bombyx mori
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DOI：
10.1007/s007050070054
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发表时间：
2000-01-01
影响因子：
2.7
通讯作者：
Maeda, S
中科院分区：
文献类型：
--
作者：
Iwanaga, M;Kang, WK;Maeda, S
研究了家蚕蛹感染杆状病毒后，对变态期脂肪体程序性降解的影响。家蚕核型多角体病毒（BmNPV）感染后，蛹脂肪体没有发生降解。感染后48 h，模拟组和BmNPV感染组的脂肪体组织之间无溶组织差异。注射后48至72小时，观察到显著差异。为了确定脂肪体的组织溶解是否是由于细胞凋亡，在不同的p.i.时间和分析。在模拟和BmNPV感染的蛹中，在化蛹后24和48 h观察到快速的基因组DNA片段化。然而，BmNPV感染明显抑制DNA片段化和梯形形成在72小时和以后的时间p.i.此外，脉冲标记分析表明，BmNPV感染恢复脂肪体细胞中的蛋白质合成。这些结果表明，在蛹-成虫发育过程中的脂肪体降解是由于细胞凋亡，BmNPV能够引起这些细胞的细胞反应。
The effect of baculovirus infection into silkworm pupa particularly on programmed fat body degradation during metamorphosis was investigated. Pupal fat body degradation did not occur following infection with Bombyx mori nucleopolyhedrovirus (BmNPV). There were no histolytic differences between the fat body tissues of mock and BmNPV infected papae until 48 h postinfection (p.i.). Between 48 and 72 h p.i., significant differences were observed. In order to determine whether the histolysis of fat body was due to apoptosis, genomic DNAs were purified at various p.i. times and analyzed. Rapid genomic DNA fragmentation was observed at 24 and 48 h after pupation in both mock and BmNPV infected pupae. However, BmNPV infection clearly inhibited DNA fragmentation and ladder formation at 72 h and later times p.i. Furthermore, pulse-labeling analysis showed that BmNPV infection restored protein synthesis in fat body cells. These results suggested that fat body degradation during pupal-adult development was due to apoptosis, and that BmNPV was able to evoke a cellular response in these cells.