ROTENONE INHIBITION OF SPINDLE MICROTUBULE ASSEMBLY IN MAMMALIAN-CELLS
ROTENONE INHIBITION OF SPINDLE MICROTUBULE ASSEMBLY IN MAMMALIAN-CELLS
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DOI：
10.1016/0014-4827(74)90210-9
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发表时间：
1974-01-01
影响因子：
3.7
通讯作者：
FULLER, GM
中科院分区：
文献类型：
--
作者：
BRINKLEY, BR;BARHAM, SS;FULLER, GM
鱼藤酮是一种有效的线粒体呼吸抑制剂，也是一种有效的抗有丝分裂剂。向指数生长的中国仓鼠卵巢细胞中添加鱼藤酮或秋水仙胺，90 分钟后导致有丝分裂指数急剧增加。当培养物洗去药物后，有丝分裂完成，细胞以大致相同的速率进展到 G 1 期。鱼藤酮阻滞细胞与秋水仙碱诱导的有丝分裂抑制的进一步相似性在超微结构水平上是明显的。用这两种药物处理的有丝分裂细胞含有单极纺锤体，其染色体聚集在细胞中心附近的中心粒对周围。在动粒和中心粒附近偶尔可见微管。这些观察结果以及鱼藤酮抑制 3 H-秋水仙碱与分离的牛脑微管蛋白结合的事实表明，鱼藤酮通过直接与微管蛋白结合以防止微管组装来抑制有丝分裂。
Rotenone, a potent inhibitor of mitochondrial respiration is also an effective antimitotic agent. The addition of either rotenone or Colcemid to exponentially growing Chinese hamster ovary cells resulted in a dramatic increase in mitotic index after 90 min. When the cultures were washed free of the drugs, mitosis was completed and the cells progressed into G 1 at approximately the same rate. Further similarity of rotenone-arrested cells to Colcemid-induced mitotic inhibition was apparent at the ultrastructural level. Mitotic cells treated by either drug contained monopolar spindles with chromosomes grouped around centriole pairs near the cell center. Occasional microtubules were seen near the kinetochore and centrioles. These observations, along with the fact that rotenone inhibited the binding of3H-colchicine to isolated bovine brain tubulin, suggested that rotenone inhibited mitosis by binding directly to tubulin to prevent microtubule assembly.