Microbeam MAD Beamline for Challenging Protein Crystallography in TPS
Microbeam MAD Beamline for Challenging Protein Crystallography in TPS
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DOI：
10.1088/1742-6596/425/1/012004
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发表时间：
2013-03
期刊：
影响因子：
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通讯作者：
D. G. Liu;C. Chao;C. H. Chang;J. Juang;C. Y. Liu;S. Chang;C. F. Chang;C. Chou;C. Tseng;C. Chiang;Y. Jean;M. Tang;S. Chung;S. Chang
中科院分区：
文献类型：
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作者：
D. G. Liu;C. Chao;C. H. Chang;J. Juang;C. Y. Liu;S. Chang;C. F. Chang;C. Chou;C. Tseng;C. Chiang;Y. Jean;M. Tang;S. Chung;S. Chang
TPS-05 A光束线是NSRRC为微量蛋白质晶体学实验建造的第一条X射线光束线，也是TPS同步加速器设施第一阶段的七条ID光束线之一。2米真空波荡器（IU 22）用作光子源，谐波#3至#9将提供1018 - 1020个光子s-1 mrad-2 mm-2的亮度（0.1%带宽）-1，光子通量为1013 - 1014光子s-1（0.1%带宽）−1，所需能量范围为5.7−20 keV（2.175−0.620 keV），以涵盖1 keV的MAD定相实验和2 keV的SAD定相实验。光束线光学系统由一个低温冷却的双晶单色器（DCM）和一对聚焦K-B镜组成。用户组的要求包括样品位置的目标焦点尺寸为50 μm × 50 μm（H × V），Se K边缘（0.9795 μ m）处的光子通量大于2 × 1012光子s-1，用于将光束尺寸调整到5 μm的针孔。本文还计算了第一光学元件，即DCM第一晶体的热负荷。
The TPS-05A beamline is the first X-ray beamline at NSRRC built for micro protein crystallography experiment as well as one of the seven ID beamlines in phase I at the TPS synchrotron facility. A 2-meter in-vacuum undulator (IU22) serves as the photon source from which the harmonics #3 to #9 will provide brilliance of 1018−1020 photons s−1 mrad−2 mm−2 (0.1% bandwidth)−1 and photon flux of 1013−1014 photons s−1 (0.1% bandwidth)−1 in the required energy range of 5.7−20 keV (2.175−0.620 Å) to cover MAD phasing experiments at 1 Å and SAD phasing experiments at 2 Å. The beamline optics consists of a cryo-cooled double crystal monochromator (DCM) and a pair of focusing K-B mirrors. Requirements from the user group include a target focus size of 50 μm × 50 μm (H × V) at the sample position, photon flux greater than 2 × 1012 photons s−1 at Se K-edge (0.9795 Å), pinholes for adjusting the beam size down to 5 μm. Calculation of heat load for the first optical element, i.e. the first crystal of DCM, is included in this paper.