HPLC analysis of isoflavonoids and other phenolic agents from foods and from human fluids
HPLC analysis of isoflavonoids and other phenolic agents from foods and from human fluids
复制标题
DOI：
10.3181/00379727-217-44231
复制
发表时间：
1998-03-01
影响因子：
--
通讯作者：
Shi, CY
中科院分区：
文献类型：
--
作者：
Franke, AA;Custer, LJ;Shi, CY
提出了一种快速、精确和选择性的二极管阵列HPLC方法，用于从食物和人尿液、血浆和母乳中提取和分析大豆黄酮，以支持评估大豆或大豆黄酮潜在癌症保护作用的机制和流行病学研究。选择固相或溶剂萃取进行分离，并根据待分析的基质使用酶或酸水解程序进行糖苷配基生产。应用C-18反相HPLC选择性分离和定量大豆苷元（1）、（2）黄豆黄素（3）、（2）和染料木素（4）、（2），包括它们的丙二酰酯（a）（2）和乙酰酯（B）（2），以及它们的哺乳动物代谢物雌马酚（6）、（2）和O-去甲基安哥林素（7）、（2）以及芒柄花黄素（2）、（2）鹰嘴豆素-A（5），（2）和香豆雌酚（8）（2）。UV吸光度扫描和真实标准品用于鉴定目的，除了荧光监测，电化学检测和GC/MS分析后，三甲基硅烷化。通过监测单个化合物的最大吸收，发现20 μ l注射液中大豆苷元、染料木黄酮、雌马酚和O-去甲基安哥拉辛（DMA）的检测限分别为1.09、0.53、3.28和1.00皮摩尔。所提出的方法被应用于监测大豆食品和人血浆，尿液和母乳中的抗氧化剂水平与烤大豆挑战后。所提出的结果的潜在活性的干扰素，以防止新生儿接触这些药物的癌症进行了讨论。
A fast, precise and selective diode array HPLC method is presented for the extraction and analysis of soy isoflavonoids from foods and from human urine, plasma, and breast milk in support of mechanistic and epidemiologic studies assessing the potential cancer protective role of soya or isoflavones. Solid phase or solvent extraction was chosen for isolation, and enzymatic or acid hydrolysis procedures were used for aglycone production depending on the matrix to be analyzed. C-18 reversed-phase HPLC was applied to selectively separate and quantitate daidzein (1),(2) glycitein (3),(2) and genistein (4),(2) including their malonyl (a)(2) and acetyl (b)(2) esters, and their mammalian metabolites equol (6)(2) and O-desmethylangolensin (7),(2) as well as formononetin (2),(2) biochanin-A (5),(2) and coumestrol (8)(2) using a gradient elution system. UV absorbance scans and authentic standards were applied for identification purposes, additional to fluorometric monitoring, electrochemical detection, and GC/MS analysis after trimethyl silylation. Detection limits of 20-mu l injections were found to be 1.09, 0.53, 3.28, and 1.00 pmoles for daidzein, genistein, equol, and O-desmethyl-angolensin (DMA), respectively, by monitoring at the individual compound's absorption maximum. The proposed method was applied to monitor isoflavone levels in soy foods and in human plasma, urine and breast milk after challenge with roasted soybeans. Implications of the presented results on the potential activity of isoflavones to prevent cancer by exposing newborn infants to these agents are discussed.