Capturing red blood cells from the blood by lectin recognition on a glycopolymer-patterned surface.
Capturing red blood cells from the blood by lectin recognition on a glycopolymer-patterned surface.
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DOI：
10.1039/c6tb00606j
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发表时间：
2016-06
期刊：
影响因子：
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通讯作者：
Chunyu Zhao;Q. Shi;Jianwen Hou;Z. Xin;Jing Jin;Chunming Li;S. Wong;Jinghua Yin
中科院分区：
文献类型：
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作者：
Chunyu Zhao;Q. Shi;Jianwen Hou;Z. Xin;Jing Jin;Chunming Li;S. Wong;Jinghua Yin
展示了一种新的用于捕获红细胞（RBC）的二元共聚物图案化表面。我们的策略是基于表面引发的光聚合的2-丙烯酰胺基-2-甲基丙磺酸（AMPS）上的热塑性弹性体，图案化的聚（d-甲基丙烯酸异丙酰胺乙酯）（PGAMA，glycopolymer）的PAMPS层上的微域与光掩模辅助光刻，然后通过生成的植物血凝素（PHA）阵列图案化的表面上通过凝集素-碳水化合物识别。我们证明了具有高横向分辨率的双组分聚合物图案化表面的成功制备;具有图案化的糖基共聚物结构域的PAMPS层保持亲水性以抵抗非特异性血浆蛋白吸附和细胞粘附;图案化的PGAMA结构域上的PHA阵列几乎不诱导血小板粘附在图案化的表面上，但显示出捕获血液中RBC的高能力，此外，捕获的RBC保持细胞的完整性和功能。我们的工作不仅为从血液中捕获红细胞铺平了新的途径，而且建立了无损伤地捕获血液或生物流体中的非粘附细胞的基本原理。
A newly glycopolymer-patterned surface for capturing red blood cells (RBCs) is demonstrated. Our strategy is based on the surface-initiated photopolymerization of 2-acryl-amido-2-methylpropane sulfonic acid (AMPS) on a thermoplastic elastomer, the patterning of poly(d-gluconamidoethyl methacrylate) (PGAMA, glycopolymer) micro-domains on the PAMPS layer with photomask-assisted photolithography, followed by the generation of a phytohemagglutinin (PHA) array on the patterned surface through lectin-carbohydrate recognition. We demonstrate that the bi-component polymer-patterned surface with high lateral resolution is successfully fabricated; the PAMPS layer with patterned glycopolymer domains remains hydrophilic to resist non-specific plasma protein adsorption and cell adhesion; the PHA array on the patterned PGAMA domains induces nearly no platelet adhesion on the patterned surface, but shows high capability for capturing RBCs in the blood, and in addition, the captured RBCs maintain cellular integrity and function. Our work presented herein not only paves a new way for capturing RBCs from the blood, but also establishes a basic principle to capture non-adherent cells in the blood or biological fluid without damage.