Identification of miRNAs encoded by Autographa californica nucleopolyhedrovirus.
Identification of miRNAs encoded by Autographa californica nucleopolyhedrovirus.
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DOI：
10.1099/jgv.0.001510
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发表时间：
2020-11
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影响因子：
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通讯作者：
Jinwen Wang;K. Xing;Peiwen Xiong;Hai Liang;Mengxiao Zhu;Jin Zhao;Xinghua Yu;Xiaolian Ning;Runcai Li;Xunzhang Wang
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文献类型：
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作者：
Jinwen Wang;K. Xing;Peiwen Xiong;Hai Liang;Mengxiao Zhu;Jin Zhao;Xinghua Yu;Xiaolian Ning;Runcai Li;Xunzhang Wang
我们分别在2013年和2019年报道了两个加州签名核多角体病毒（AcMNPV）编码的miRNAs， AcMNPV- mir -1和AcMNPV- mir -3。在这里，我们对acmnpv编码的mirna进行了综合研究，其中包括上述两个mirna和另外三个新发现的mirna。通过小RNA深度测序和生物信息学预测了6个候选mirna，其中5个得到了验证。三个mirna位于编码序列的对面，另外两个位于病毒基因的编码序列中。预测病毒和宿主的靶标，随后使用双荧光素酶报告基因测定法进行检测。除了AcMNPV- mir -4靶标均为宿主基因外，验证的靶标主要存在于AcMNPV中。根据报告基因分析，这5种mirna主要通过下调它们的靶标来发挥作用。这些mirna的转录起始位点是基于已知的杆状病毒启动子基序进行生物信息学筛选的。我们的研究表明，acmnpv编码的miRNAs通过调节病毒和/或宿主基因，作为宿主与病毒相互作用的精细调节剂。
Two Autographa californica nucleopolyhedrovirus (AcMNPV) encoded miRNAs, AcMNPV-miR-1 and AcMNPV-miR-3, have been reported by us in 2013 and 2019, respectively. Here, we present an integrated investigation of AcMNPV-encoded miRNAs, which include the above two miRNAs and three additional newly identified miRNAs. Six candidate miRNAs were predicted through small RNA deep sequencing and bioinformatics, of which, five were validated. Three miRNAs are located opposite the coding sequences, the other two are located in the coding sequences of viral genes. Targets in both virus and host were predicted and subsequently tested using dual-luciferase reporter assays. The validated targets were found mainly in AcMNPV, except for the targets of AcMNPV-miR-4, which are all host genes. Based on reporter assays, the five miRNAs predominantly function by down-regulating their targets. The transcription start sites of these miRNAs were bioinformatic screened based on known baculovirus promoter motifs. Our study reveals that AcMNPV-encoded miRNAs function as fine modulators of the interactions between host and virus by regulating viral and/or host genes.