Fiber-Based Mini Tissue with Morphology-Controllable GelMA Microfibers
Fiber-Based Mini Tissue with Morphology-Controllable GelMA Microfibers
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DOI：
10.1002/smll.201802187
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发表时间：
2018-11-02
期刊：
影响因子：
13.3
通讯作者：
He, Yong
中科院分区：
文献类型：
--
作者：
Shao, Lei;Gao, Qing;He, Yong
在基于纤维的组织工程中，使用微尺度纤维可以促进营养物质的扩散，提高细胞存活率。然而，为了构建肌肉纤维、神经导管和血管等功能性微型组织，水凝胶微纤维不仅要模仿天然组织的结构特征，而且要为组织功能化提供有利的细胞环境和足够的强度。因此，一个重要的目标是用合适的水凝胶材料来制备形态可控的微纤维，以模拟天然组织的结构和功能的复杂性。本文以具有优良生物性能的甲基丙烯酸明胶(GelMA)为纤维材料，开发了一种新型的同轴生物印花方法来制备海藻酸钙包裹的形态可控的GelMA微纤维。通过调节流量，可以得到直线型、波浪型和螺旋型的GelMA超细纤维。通过改变同轴喷嘴的设计，可以制备出更复杂的GelMA微纤维，如Janus、多层和双螺旋结构。使用这些微纤维，可以构建含有人脐静脉内皮细胞的微型组织，细胞在其中逐渐迁移和连接，形成类似血管的管腔。多功能超细纤维的细胞相容性、结构多样性和机械可调谐性的优点可能为进一步的生物医学研究开辟更多的途径。
The use of microscale fibers could facilitate nutrient diffusion in fiber-based tissue engineering and improve cell survival. However, in order to build a functional mini tissue such as muscle fibers, nerve conduits, and blood vessels, hydrogel microfibers should not only mimic the structural features of native tissues but also offer a cell-favorable environment and sufficient strength for tissue functionalization. Therefore, an important goal is to fabricate morphology-controllable microfibers with appropriate hydrogel materials to mimic the structural and functional complexity of native tissues. Here, gelatin methacrylate (GelMA) is used as the fiber material due to its excellent biological performance, and a novel coaxial bioprinting method is developed to fabricate morphology-controllable GelMA microfibers encapsulated in calcium alginate. By adjusting the flow rates, GelMA microfibers with straight, wavy, and helical morphologies could be obtained. By varying the coaxial nozzle design, more complex GelMA microfibers such as Janus, multilayered, and double helix structures could be fabricated. Using these microfibers, mini tissues containing human umbilical cord vein endothelial cells are built, in which cells gradually migrate and connect to form lumen resembling blood vessels. The merits of cytocompatibility, structural diversity, and mechanical tunability of the versatile microfibers may open more avenues for further biomedical research.