Evaluation of inactivation methods for severe acute respiratory syndrome coronavirus in noncellular blood products
Evaluation of inactivation methods for severe acute respiratory syndrome coronavirus in noncellular blood products
复制标题
DOI：
10.1111/j.1537-2995.2006.00976.x
复制
发表时间：
2006-10-01
期刊：
影响因子：
2.9
通讯作者：
Taylor, Deborah R.
中科院分区：
文献类型：
--
作者：
Darnell, Miriam E. R.;Taylor, Deborah R.
严重急性呼吸综合征冠状病毒（SARS-CoV）已在感染者的血液中检测到，在未来爆发疫情时，可能会污染捐献的血液和血浆衍生产品。本报告描述了灭活蛋白质溶液中SARS-CoV的有效方法，分别测试了热、紫外线（UV）照射、辛酸和溶剂/洗涤剂（S/D）方法在适当模拟血液衍生产品的蛋白质溶液中灭活SARS-CoV的能力。在组织培养生长试验中测试处理过的样品的灭活情况。在60摄氏度下热处理病毒灭活需要15到30分钟才能灭活SARS冠状病毒。UVC在40分钟内有效地灭活了SARS-CoV，而UVA需要添加peptide来增强病毒的灭活。牛血清白蛋白的存在限制了UVC和UVA抑制SARS-CoV的能力，辛酸处理不会降低SARS-CoV加标蛋白溶液的感染性。Triton X-100、Tween 80和胆酸钠的S/D处理分别需要2、4和长达24 h才能灭活SARS-CoV，热、UVC照射和S/D处理可有效灭活SARS-CoV，而辛酸处理不足以灭活病毒。
Severe acute respiratory syndrome coronavirus (SARS-CoV) has been detected in the blood of infected individuals, which may have the potential to contaminate donated blood and plasma-derived products in the event of a future outbreak. Effective methods for inactivating the SARS-CoV in protein solutions are described in this report.Heat, ultraviolet (UV) irradiation, octanoic acid, and solvent/detergent (S/D) methods were tested individually for their ability to inactivate SARS-CoV in protein solutions appropriately mimicking blood-derived products. Treated samples were tested for inactivation in a tissue culture growth assay.Viral inactivation by heat treatment at 60 degrees C required 15 to 30 minutes to inactivate the SARS-CoV. UVC efficiently inactivated SARS-CoV in 40 minutes, whereas UVA required the addition of psoralen to enhance inactivation of the virus. The presence of bovine serum albumin limited the ability of UVC and UVA to inactivate SARS-CoV and octanoic acid treatment does not reduce the infectivity of SARS-CoV-spiked protein solutions. S/D treatment required 2, 4, and up to 24 hours for Triton X-100, Tween 80, and sodium cholate inactivation, respectively.Heat, UVC irradiation, and S/D treatments effectively inactivate SARS-CoV, whereas octanoic acid treatment is insufficient for inactivation of the virus.