MEASUREMENT OF PROTEIN TURNOVER IN RAT-LIVER - ANALYSIS OF COMPLEX CURVE FOR DECAY OF LABEL IN A MIXTURE OF PROTEINS
MEASUREMENT OF PROTEIN TURNOVER IN RAT-LIVER - ANALYSIS OF COMPLEX CURVE FOR DECAY OF LABEL IN A MIXTURE OF PROTEINS
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DOI：
10.1042/bj1560657
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发表时间：
1976-01-01
影响因子：
4.1
通讯作者：
SWICK, RW
中科院分区：
文献类型：
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作者：
GARLICK, PJ;WATERLOW, JC;SWICK, RW
分析注射NaH_（14）CO_3标记的大鼠肝蛋白中~（14）C的衰变曲线，得到混合肝蛋白的平均周转率。使用了三种不同的方法。与来自同质蛋白质的衰减曲线不同，该曲线不拟合单个指数，而是用3个指数获得良好的拟合。通过假设混合物含有3种具有不同周转率的主要组分，平均周转率（k）的计算值接近40% /天; k也是根据衰变曲线下的面积计算的，这种方法不假设混合物中蛋白质的数量。该方法也给出了接近40%/天的值。经验表明，通过刺激蛋白质模型混合物中标记的衰减和体内测量的衰减曲线，可以从比放射性下降到其最大值的10%所需的时间计算k。这是一个优点，因为其他两种方法需要在更长的时间段内测量衰减曲线。
The curve for decay of 14C in rat liver protein labeled by injection of NaH14CO3 was analyzed to obtain the average turnover rate of mixed liver protein. Three different methods were used. Unlike decay curves from homogeneous proteins, the curve did not fit a single exponential, but a good fit was obtained with 3 exponentials. By assuming that the mixture contained 3 major components with different turnover rates, the calculated value for the average turnover rate (k) was close to 40% /day; k was also calculated from the area under the decay curve, a method which makes no assumptions about the number of proteins in the mixture. This method also gave a value close to 40%/day. It was shown empirically, by stimulation of decay of label in model mixtures of protein and with the decay curve measured in vivo, that k can be calculated from the time taken for specific radioactivity to fall to 10% of its maximum value. This is an advantage, since the other 2 methods require the decay curve to be measured over a much longer period of time.