Screening of tropically derived, multi-trait plant growth- promoting rhizobacteria and evaluation of corn and soybean colonization ability
Screening of tropically derived, multi-trait plant growth- promoting rhizobacteria and evaluation of corn and soybean colonization ability
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DOI：
10.1016/j.micres.2017.09.007
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发表时间：
2018-01-01
影响因子：
6.7
通讯作者：
Quecine, Maria Carolina
中科院分区：
文献类型：
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作者：
Batista, Bruna Durante;Lacava, Paulo Teixeira;Quecine, Maria Carolina
本研究对典型的热带植物泡球兰（Paullinia cupana）的促生特性和可培养根际细菌多样性进行了研究。96个细菌分离，进行生化试验，并确定了部分或全部16S rDNA测序。变形菌门和厚壁菌门是根际优势菌门，伯克霍尔德菌属和芽孢杆菌属是最丰富的属。13个菌株具有评价的4个PGP性状，其中大多数属于伯克霍尔德氏菌属。两株多性状PGP菌株RZ2MS9（Bacillus sp.）和RZ2MS 16（Burkholderia ambifaria）在温室条件下显著促进玉米和大豆生长。与未接种对照相比，接种RZ2MS 9和RZ2MS 16的玉米根干重分别增加247.8%和136.9%。玉米和大豆苗的干重显著高于未接种的植株，菌株和作物的干重均增加了47%以上。然而，大豆根干重没有增加细菌接种后与任何菌株。使用GFP标记结合荧光显微镜和基于培养的定量方法评估RZ2MS 16的定殖行为。RZ2MS 16：gfp能够定殖于玉米和大豆的根和芽，揭示了内生行为。
The present study assessed the plant growth-promoting (PGP) traits and diversity of culturable rhizobacteria associated with guarana (Paullinia cupana), a typical tropical plant. Ninety-six bacteria were isolated, subjected to biochemical tests, and identified by partial or total 16S rDNA sequencing. Proteobacteria and Firmicutes were the dominant rhizospheric phyla found, and Burkholderia and Bacillus were the most abundant genera. Thirteen strains exhibited the four PGP traits evaluated, and most of them belonged to the genus Burkholderia. Two multi trait PGP strains, RZ2MS9 (Bacillus sp.) and RZ2MS16 (Burkholderia ambifaria), expressively promoted corn and soybean growth under greenhouse conditions. Compared to the non-inoculated control, increases in corn root dry weight of 247.8 and 136.9% were obtained with RZ2MS9 and RZ2MS16 inoculation, respectively, at 60 days after seeding. The dry weights of corn and soybean shoots were significantly higher than those of non-inoculated plants, showing increases of more than 47% for both strains and crops. However, soybean root dry weight did not increased after bacterial inoculation with either strain. The colonization behavior of RZ2MS16 was assessed using GFP-labeling combined with fluorescence microscopy and a cultivation-based approach for quantification. RZ2MS16:gfp was able to colonize the roots and shoots of corn and soybean, revealing an endophytic behavior.