Quantitation of pyrimidine dimers by immunoslot blot following sublethal UV-irradiation of human cells.
Quantitation of pyrimidine dimers by immunoslot blot following sublethal UV-irradiation of human cells.
复制标题
对人体细胞进行亚致死紫外线照射后，通过免疫印迹法对嘧啶二聚体进行定量。
DOI：
10.1111/j.1751-1097.1987.tb04798.x
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发表时间：
1987
影响因子：
3.3
通讯作者：
Alvi,NK
中科院分区：
文献类型：
--
作者：
Wani,AA;D'Ambrosio,SM;Alvi,NK
An immunoslot blot assay was developed to detect pyrimidine dimers induced in DNA by sublethal doses of UV (254 nm) radiation. Using this assay, one dimer could be detected in 10 megabase DNA using 200 ng or 0.5 megabase DNA using 20 ng irradiated DNA. The level of detection, as measured by dimer specific antibody binding, was proportional to the dose of UV and amount of irradiated DNA used. The repair of pyrimidine dimers was measured in human skin fibroblastic cells in culture following exposure to 0.5 to 5 J m‐2of 254 nm UV radiation. The half‐life of repair was approximately 24, 7 and 6 h in cells exposed to 0.5, 2 and 5 J m‐2UV radiation, respectively. This immunological approach utilizing irradiated DNA immobilized to nitrocellulose should allow the direct quantitation of dimers following very low levels of irradiation in small biological samples and isolated gene fragments.
