Efficient enzymatic synthesis of LNA-modified DNA duplexes using KOD DNA polymerase
Efficient enzymatic synthesis of LNA-modified DNA duplexes using KOD DNA polymerase
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DOI：
10.1039/b819946a
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发表时间：
2009-01-01
影响因子：
3.2
通讯作者：
Wengel, Jesper
中科院分区：
文献类型：
--
作者：
Veedu, Rakesh N.;Vester, Birte;Wengel, Jesper
以LNA-TTP、LNA-ATP和LNA-5-甲基-CTP三种不同的LNA-核苷三磷酸作为KOD DNA聚合酶的底物进行了研究。结果表明，KOD DNA聚合酶是一种有效的催化剂，可用于模板导向的DNA寡核苷酸双链的模板延伸反应。此外，KOD DNA聚合酶适用于LNA修饰的DNA双链的PCR扩增。
Three different LNA-nucleoside triphosphates, LNA-TTP, LNA-ATP and LNA-5-methyl-CTP, were investigated as substrates for KOD DNA polymerase. The results reveal that KOD DNA polymerase is an efficient catalyst for template directed synthesis of DNA oligonucleotide duplexes containing a large number of LNA nucleotides by primer extension reactions. Furthermore, KOD DNA polymerase is shown to be suitable for the PCR amplification of an LNA-modified DNA duplex.