Activation in vagal afferents and central autonomic pathways:: Early responses to intestinal infection with Campylobacter jejuni
Activation in vagal afferents and central autonomic pathways:: Early responses to intestinal infection with Campylobacter jejuni
复制标题
DOI：
10.1016/j.bbi.2004.09.002
复制
发表时间：
2005-07-01
影响因子：
15.1
通讯作者：
Lyte, M
中科院分区：
文献类型：
--
作者：
Goehler, LE;Gaykema, RPA;Lyte, M
现在有大量证据支持这样一种观点，即免疫系统与大脑之间的交流有多种途径或机制。各种机制的存在表明，它们可能各自对免疫感觉信号传导有不同的贡献。例如，脑介导的免疫信号转导依赖于循环介质的存在，而周围感觉神经更可能在感染的早期，在循环细胞因子升高之前，或在这些神经终末区的局部感染中发挥重要作用。为了验证肠道局部感染激活迷走神经感觉神经元的假设，我们在口服生理盐水或活空肠弯曲杆菌（C jejuni）小鼠中，评估了迷走神经感觉神经节神经元和迷走神经初级感觉传递核（孤立束核）神经元中神经元激活标记物C - fos的表达。用C -空肠或生理盐水分别接种雄性CF1小鼠，免疫组化检测4-12 h后迷走神经感觉神经元和大脑中C - fos的表达。在循环促炎细胞因子水平未升高的情况下，口服空肠C导致迷走神经节双侧神经元中C - fos表达显著增加。C -空肠处理激活了nTS中的神经元，以及与初级内脏感觉通路和中枢自主神经网络相关的大脑区域。这些发现提供了证据，表明外周感觉神经元有助于向大脑提供有关潜在病原体的早期信号。(c) 2004 Elsevier Inc.版权所有。
Abundant evidence now supports the idea that multiple pathways or mechanisms underlie communication from the immune system to the brain. The presence of a variety of mechanisms suggests that they may each contribute something different to immunosensory signaling. For instance, brain mediated immune signal transduction is dependent upon the presence of circulating mediators whereas peripheral sensory nerves are more likely to be important early on in an infection, prior to elevation of circulating cytokines, or in local infections within the terminal fields of these nerves. To test the hypothesis that local infection in the gut activates vagal sensory neurons, we assessed expression of the neuronal activation marker c-Fos in neurons in the vagal sensory ganglia and in the primary sensory relay nucleus for the vagus, the nucleus of the solitary tract (nTS) in mice treated orally either with saline or live Campylobacter jejuni (C jejuni). Male CF1 mice were inoculated orally with either C jejuni or saline, and c-Fos expression in the vagal sensory neurons and brain 4-12 h later was assessed via immunohistochemistry. Oral inoculation with C jejuni led to a significant increase in c-Fos expression in neurons bilaterally in the vagal ganglia, in the absence of elevated levels of circulating proinflammatory cytokines. C jejuni treatment activated neurons in the nTS, as well as in brain regions associated with primary viscerosensory pathways and the central autonomic network. These findings provide evidence that peripheral sensory neurons contribute an early signal to the brain regarding potential pathogens. (c) 2004 Elsevier Inc. All rights reserved.