Antigenic differences detected between prespore cells of Dictyostelium discoideum and Dictyostelium mucoroides using monoclonal antibodies.
Antigenic differences detected between prespore cells of Dictyostelium discoideum and Dictyostelium mucoroides using monoclonal antibodies.
复制标题
使用单克隆抗体检测盘基网柄菌和粘液盘基网柄菌的前孢子细胞之间的抗原差异。
DOI：
10.1016/0014-4827(82)90427-x
复制
发表时间：
1982
影响因子：
3.7
通讯作者：
Williams,KL
中科院分区：
文献类型：
--
作者：
Gregg,JH;Krefft,M;Haas-Kraus,A;Williams,KL
建立了一种获得多聚甲醛固定的竹鞭毛虫冰冻切片的方法。这些切片是使用单抗进行研究的理想选择。用两种不同的单抗识别牙周膜表面的特异性抗原。荧光显微镜下观察盘状大孢子细胞。两株单抗均未与TOD结合。圆盘状茎前柄细胞.D。Discoideum不能将任何一种单抗与其前孢子细胞结合。这些结果与D或D的结果不同。Mororoids[1]Ord.Discoideum[2]多特异性抗血清。
A method was developed to obtain frozen sections of paraformaldehyde-fixedDictyosteliumslugs. These sections were ideal for studies using monoclonal antibodies. Two different monoclonal antibodies were used to recognize specific antigens on the surface ofD. discoideumprespore cells by fluorescence microscopy. Neither of the two monoclonal antibodies bound toD. discoideumprestalk cells.D. mucoroides, a closely related species toD. discoideum, failed to bind either monoclonal antibody to its prespore cells. These results contrast with those obtained with eitherD. mucoroides[1] orD. discoideum[2] polyspecific antisera.